A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer

Carlos Perez-Stable, Alicia De Las Pozas, Bernard A. Roos

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)43-53
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume167
Issue number1-2
DOIs
StatePublished - Sep 25 2000

Fingerprint

GATA Transcription Factors
Prostate-Specific Antigen
Androgens
Genes
Prostate
Globins
Polyomavirus Transforming Antigens
Antigen Receptors
Androgen Receptors
Response Elements
Transgenic Mice
Prostatic Neoplasms
Plasmids
Transcription Factors
Cells
Carcinoma
Cell Line
Messenger RNA

Keywords

  • Dihydrotestosterone
  • Electrophoretic mobility shift assay
  • GATA family
  • LNCaP
  • PSA enhancer
  • Transfection

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer. / Perez-Stable, Carlos; Pozas, Alicia De Las; Roos, Bernard A.

In: Molecular and Cellular Endocrinology, Vol. 167, No. 1-2, 25.09.2000, p. 43-53.

Research output: Contribution to journalArticle

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