Abstract
The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 43-53 |
| Number of pages | 11 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 167 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 25 2000 |
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Keywords
- Dihydrotestosterone
- Electrophoretic mobility shift assay
- GATA family
- LNCaP
- PSA enhancer
- Transfection
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer. / Perez-Stable, Carlos; Pozas, Alicia De Las; Roos, Bernard A.
In: Molecular and Cellular Endocrinology, Vol. 167, No. 1-2, 25.09.2000, p. 43-53.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer
AU - Perez-Stable, Carlos
AU - Pozas, Alicia De Las
AU - Roos, Bernard A.
PY - 2000/9/25
Y1 - 2000/9/25
N2 - The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.
AB - The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.
KW - Dihydrotestosterone
KW - Electrophoretic mobility shift assay
KW - GATA family
KW - LNCaP
KW - PSA enhancer
KW - Transfection
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UR - http://www.scopus.com/inward/citedby.url?scp=0343714601&partnerID=8YFLogxK
U2 - 10.1016/S0303-7207(00)00300-2
DO - 10.1016/S0303-7207(00)00300-2
M3 - Article
C2 - 11000519
AN - SCOPUS:0343714601
VL - 167
SP - 43
EP - 53
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 1-2
ER -