A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer

Carlos M. Perez-Stable; Alicia De Las Pozas; Bernard A. Roos

doi:10.1016/S0303-7207(00)00300-2

A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer

Carlos M. Perez-Stable, Alicia De Las Pozas, Bernard A. Roos

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	43-53
Number of pages	11
Journal	Molecular and Cellular Endocrinology
Volume	167
Issue number	1-2
DOIs	https://doi.org/10.1016/S0303-7207(00)00300-2
State	Published - Sep 25 2000
Externally published	Yes

Keywords

Dihydrotestosterone
Electrophoretic mobility shift assay
GATA family
LNCaP
PSA enhancer
Transfection

ASJC Scopus subject areas

Endocrinology
Endocrinology, Diabetes and Metabolism

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/S0303-7207(00)00300-2

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title = "A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer",

abstract = "The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Dihydrotestosterone, Electrophoretic mobility shift assay, GATA family, LNCaP, PSA enhancer, Transfection",

author = "Perez-Stable, {Carlos M.} and Pozas, {Alicia De Las} and Roos, {Bernard A.}",

note = "Funding Information: We thank the two anonymous reviewers for their thoughtful comments; Dr Kerry Burnstein and Dr Parmender Mehta for critical reading of the manuscript; Dr Ed Oates for design of primers for RT-PCR; Adan Farinas for technical assistance; and Virginia Roos for help in preparing the manuscript. This work was supported in part by a Department of Defense grant (DAMD17-98-1-8525) and a National Cancer Institute Center for Psycho-Oncology grant (1P50CA84944).",

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AU - Perez-Stable, Carlos M.

AU - Pozas, Alicia De Las

AU - Roos, Bernard A.

N1 - Funding Information: We thank the two anonymous reviewers for their thoughtful comments; Dr Kerry Burnstein and Dr Parmender Mehta for critical reading of the manuscript; Dr Ed Oates for design of primers for RT-PCR; Adan Farinas for technical assistance; and Virginia Roos for help in preparing the manuscript. This work was supported in part by a Department of Defense grant (DAMD17-98-1-8525) and a National Cancer Institute Center for Psycho-Oncology grant (1P50CA84944).

PY - 2000/9/25

Y1 - 2000/9/25

N2 - The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.

AB - The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.

KW - Dihydrotestosterone

KW - Electrophoretic mobility shift assay

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KW - LNCaP

KW - PSA enhancer

KW - Transfection

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A role for GATA transcription factors in the androgen regulation of the prostate-specific antigen gene enhancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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