Abstract
The transgenic mouse line Gγ/T-15 containing the fetal globin promoter linked to SV40 T antigen unexpectedly results in androgen-independent prostate carcinomas. Given the key role of GATA-1 transcription factor in fetal globin gene promoter activity, we investigated whether specific GATA family members are expressed in the prostate and whether they can regulate prostate-specific genes. We found that GATA-2 and -3 are the predominant GATA family members expressed in human and mouse prostate and that GATA mRNA levels are not regulated by androgen. We identified six GATA sites flanking an androgen-response element located in the far-upstream enhancer of the prostate-specific antigen (PSA) gene. These GATA sites are targets for GATA factors and are essential for optimal androgen induction of transfected PSA enhancer/promoter plasmids in LNCaP, a PSA and androgen receptor expressing human prostate cancer cell line. Our results suggest that prostatic GATA-2 and -3 are involved in the androgen regulation of the PSA gene. (C) 2000 Elsevier Science Ireland Ltd.
Original language | English (US) |
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Pages (from-to) | 43-53 |
Number of pages | 11 |
Journal | Molecular and Cellular Endocrinology |
Volume | 167 |
Issue number | 1-2 |
DOIs | |
State | Published - Sep 25 2000 |
Keywords
- Dihydrotestosterone
- Electrophoretic mobility shift assay
- GATA family
- LNCaP
- PSA enhancer
- Transfection
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism