A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival

Ling Tang, Tengjiao Cui, James J. Wu, Wen Liu-Mares, Ning Huang, Jie Li

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferation. To explore the role of hLF in wound healing, we used recombinant human lactoferrin (holo-rhLF), derived from transgenic rice, to examine the effects of holo-rhLF on cell proliferation, migration, attachment, and survival in a human primary skin fibroblast culture system. This study revealed that holo-rhLF not only significantly stimulates fibroblast proliferation but also has synergistic effects with fibroblast growth factor-2 and antagonistic effects with transforming growth factor-β1 on cell proliferation. Furthermore, using a chamber migration assay, our results demonstrate that holo-rhLF promotes fibroblast migration in a dosage-dependent manner. More importantly, holo-rhLF significantly increased cell viability and protected cells from death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant effect was observed on cell attachment. In conclusion, these findings reveal the multiple functions of holo-rhLF in human skin fibroblasts and indicate its potential application in wound therapy by enhancing cell proliferation and migration as well as protecting cells from apoptosis.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalWound Repair and Regeneration
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Lactoferrin
Cell Survival
Fibroblasts
Cell Proliferation
Cell Movement
Skin
Wounds and Injuries
Transforming Growth Factors
Tetradecanoylphorbol Acetate
Fibroblast Growth Factor 2
Transferrin
Wound Healing
Glycoproteins
Cell Death
Oryza
Apoptosis
Inflammation
Cell Line
Survival
Serum

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival. / Tang, Ling; Cui, Tengjiao; Wu, James J.; Liu-Mares, Wen; Huang, Ning; Li, Jie.

In: Wound Repair and Regeneration, Vol. 18, No. 1, 01.01.2010, p. 123-131.

Research output: Contribution to journalArticle

@article{add0cf3dbd294a7ead7e47e71ee1c40f,
title = "A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival",
abstract = "Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferation. To explore the role of hLF in wound healing, we used recombinant human lactoferrin (holo-rhLF), derived from transgenic rice, to examine the effects of holo-rhLF on cell proliferation, migration, attachment, and survival in a human primary skin fibroblast culture system. This study revealed that holo-rhLF not only significantly stimulates fibroblast proliferation but also has synergistic effects with fibroblast growth factor-2 and antagonistic effects with transforming growth factor-β1 on cell proliferation. Furthermore, using a chamber migration assay, our results demonstrate that holo-rhLF promotes fibroblast migration in a dosage-dependent manner. More importantly, holo-rhLF significantly increased cell viability and protected cells from death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant effect was observed on cell attachment. In conclusion, these findings reveal the multiple functions of holo-rhLF in human skin fibroblasts and indicate its potential application in wound therapy by enhancing cell proliferation and migration as well as protecting cells from apoptosis.",
author = "Ling Tang and Tengjiao Cui and Wu, {James J.} and Wen Liu-Mares and Ning Huang and Jie Li",
year = "2010",
month = "1",
day = "1",
doi = "10.1111/j.1524-475X.2009.00563.x",
language = "English",
volume = "18",
pages = "123--131",
journal = "Wound Repair and Regeneration",
issn = "1067-1927",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - A rice-derived recombinant human lactoferrin stimulates fibroblast proliferation, migration, and sustains cell survival

AU - Tang, Ling

AU - Cui, Tengjiao

AU - Wu, James J.

AU - Liu-Mares, Wen

AU - Huang, Ning

AU - Li, Jie

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferation. To explore the role of hLF in wound healing, we used recombinant human lactoferrin (holo-rhLF), derived from transgenic rice, to examine the effects of holo-rhLF on cell proliferation, migration, attachment, and survival in a human primary skin fibroblast culture system. This study revealed that holo-rhLF not only significantly stimulates fibroblast proliferation but also has synergistic effects with fibroblast growth factor-2 and antagonistic effects with transforming growth factor-β1 on cell proliferation. Furthermore, using a chamber migration assay, our results demonstrate that holo-rhLF promotes fibroblast migration in a dosage-dependent manner. More importantly, holo-rhLF significantly increased cell viability and protected cells from death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant effect was observed on cell attachment. In conclusion, these findings reveal the multiple functions of holo-rhLF in human skin fibroblasts and indicate its potential application in wound therapy by enhancing cell proliferation and migration as well as protecting cells from apoptosis.

AB - Human lactoferrin (hLF), a glycoprotein of the transferrin family, has recently been shown to stimulate wound repair through its antimicrobial effect and inflammation modulation. A recent study with several non-skin cell lines indicated that hLF may also have a stimulatory effect on cell proliferation. To explore the role of hLF in wound healing, we used recombinant human lactoferrin (holo-rhLF), derived from transgenic rice, to examine the effects of holo-rhLF on cell proliferation, migration, attachment, and survival in a human primary skin fibroblast culture system. This study revealed that holo-rhLF not only significantly stimulates fibroblast proliferation but also has synergistic effects with fibroblast growth factor-2 and antagonistic effects with transforming growth factor-β1 on cell proliferation. Furthermore, using a chamber migration assay, our results demonstrate that holo-rhLF promotes fibroblast migration in a dosage-dependent manner. More importantly, holo-rhLF significantly increased cell viability and protected cells from death when they were stressed by either serum depletion or 12-O-tetradecanoylphorbol-13-acetate exposure. No significant effect was observed on cell attachment. In conclusion, these findings reveal the multiple functions of holo-rhLF in human skin fibroblasts and indicate its potential application in wound therapy by enhancing cell proliferation and migration as well as protecting cells from apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=74549124200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74549124200&partnerID=8YFLogxK

U2 - 10.1111/j.1524-475X.2009.00563.x

DO - 10.1111/j.1524-475X.2009.00563.x

M3 - Article

VL - 18

SP - 123

EP - 131

JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

SN - 1067-1927

IS - 1

ER -