A requirement for rac1 in the PDGF-stimulated migration of fibroblasts and vascular smooth cells

A. Masharn Doanes, Kaikobad Irani, Pascal Goldschmidt-Clermont, Toren Finkel

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Rac1 is a member of the Rho family of small GTPases. Although rac1 has been demonstrated to regulate the cytoskeleton, relatively little is known regarding its role in directional migration of mammalian cells. To address this issue, we have used recombinant adenoviruses to transiently express in fibroblasts either a dominant negative (N17rac1) or constitutively active (V12rac1) isoform of the small GTP-binding protein rac1. Expression of N17rac1 is demonstrated to inhibit PDGF-stimulated migration of rat fibroblasts. Surprisingly, expression of V12rac1 also inhibited, albeit to a lesser degree, the chemotactic response to PDGF. In contrast, expression of N17rac1 had no effect on PDGF stimulation of mitogen activated protein kinase (MAPK) or the adherence of cells to plastic or fibronectin coated surfaces. Similar to what was observed in fibroblasts, expression of N17rac1 inhibited the PDGF-stimulated migration of primary vascular smooth muscle cells. These results identify rac1 as an important downstream mediator of PDGF-induced chemotaxis.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume45
Issue number2
StatePublished - Jun 1 1998
Externally publishedYes

Fingerprint

Fibroblasts
Blood Vessels
rac1 GTP-Binding Protein
Cells
Monomeric GTP-Binding Proteins
Chemotaxis
Mitogen-Activated Protein Kinases
Cytoskeleton
Vascular Smooth Muscle
Fibronectins
Adenoviridae
Plastics
Smooth Muscle Myocytes
Cell Movement
Muscle
Rats
Protein Isoforms

Keywords

  • Adenovirus
  • Migration
  • PDGF
  • Rac1
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

A requirement for rac1 in the PDGF-stimulated migration of fibroblasts and vascular smooth cells. / Doanes, A. Masharn; Irani, Kaikobad; Goldschmidt-Clermont, Pascal; Finkel, Toren.

In: Biochemistry and Molecular Biology International, Vol. 45, No. 2, 01.06.1998, p. 279-287.

Research output: Contribution to journalArticle

Doanes, AM, Irani, K, Goldschmidt-Clermont, P & Finkel, T 1998, 'A requirement for rac1 in the PDGF-stimulated migration of fibroblasts and vascular smooth cells', Biochemistry and Molecular Biology International, vol. 45, no. 2, pp. 279-287.
Doanes, A. Masharn ; Irani, Kaikobad ; Goldschmidt-Clermont, Pascal ; Finkel, Toren. / A requirement for rac1 in the PDGF-stimulated migration of fibroblasts and vascular smooth cells. In: Biochemistry and Molecular Biology International. 1998 ; Vol. 45, No. 2. pp. 279-287.
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