A reliable and reproducible technique for DNA fingerprinting in biorepositories: A pilot study from BioBIM

Raffaele Palmirotta, Maria Laura De Marchis, Giorgia Ludovici, Cristiano Ialongo, Barbara Leone, Nadia Lopez, Maria Giovanna Valente, Antonella Spila, Patrizia Ferroni, David Della-Morte, Fiorella Guadagni

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Standard operating procedures (SOPs) optimization for nucleic acid extraction from stored samples is of crucial importance in a biological repository, considering the large number of collected samples and their future downstream molecular and biological applications. However, the validity of molecular studies using stored specimens depends not only on the integrity of the biological samples, but also on the procedures that ensure the traceability of the same sample, certifying its uniqueness, and ensuring the identification of potential sample contaminations. With this aim, we have developed a rapid, reliable, low-cost, and simple DNA fingerprinting tool for a routine use in quality control of biorepositories samples. The method consists of a double ALU insertion/deletion genotyping panel suitable for uniqueness, identification of sample contaminations, and gender validation. Preliminary data suggest that this easy-to-use DNA fingerprinting protocol could routinely provide assurances of DNA identity and quality in a biorepository setting.

Original languageEnglish (US)
Pages (from-to)e398-e404
JournalInternational Journal of Biological Markers
Volume28
Issue number4
DOIs
StatePublished - Oct 1 2013

Keywords

  • Biobank
  • Biospecimens
  • DNA fingerprint

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

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    Palmirotta, R., De Marchis, M. L., Ludovici, G., Ialongo, C., Leone, B., Lopez, N., Valente, M. G., Spila, A., Ferroni, P., Della-Morte, D., & Guadagni, F. (2013). A reliable and reproducible technique for DNA fingerprinting in biorepositories: A pilot study from BioBIM. International Journal of Biological Markers, 28(4), e398-e404. https://doi.org/10.5301/JBM.5000044