A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration

Giorgia Totonelli, Panagiotis Maghsoudlou, Massimo Garriboli, Johannes Riegler, Giuseppe Orlando, Alan J. Burns, Neil J. Sebire, Virpi V. Smith, Jonathan M. Fishman, Marco Ghionzoli, Mark Turmaine, Martin A. Birchall, Anthony Atala, Shay Soker, Mark F. Lythgoe, Alexander Seifalian, Agostino Pierro, Simon Eaton, Paolo De Coppi

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.

Original languageEnglish (US)
Pages (from-to)3401-3410
Number of pages10
JournalBiomaterials
Volume33
Issue number12
DOIs
StatePublished - Apr 2012

Keywords

  • Decellularization
  • Gut transplantation
  • Intestinal failure
  • Natural acellular matrix
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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