A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo

Joseph C. LaManna, Solomon M. Pikarsky, Thomas Sick, Myron Rosenthal

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.

Original languageEnglish
Pages (from-to)483-493
Number of pages11
JournalAnalytical Biochemistry
Volume144
Issue number2
DOIs
StatePublished - Feb 1 1985

Fingerprint

Spectrophotometers
Tissue
Scanning
Cell Physiological Phenomena
Optical Devices
Biochemistry
Physiology
Cytochromes
Light scattering
Biological materials
Light absorption
Light
Oxidation
Molecules
Kinetics
In Vitro Techniques

Keywords

  • cytochromes
  • mitochondrial redox state
  • optical methods
  • oxidative metabolism
  • rapid-scanning spectrophotometry
  • rat hippocampal slice

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo. / LaManna, Joseph C.; Pikarsky, Solomon M.; Sick, Thomas; Rosenthal, Myron.

In: Analytical Biochemistry, Vol. 144, No. 2, 01.02.1985, p. 483-493.

Research output: Contribution to journalArticle

LaManna, Joseph C. ; Pikarsky, Solomon M. ; Sick, Thomas ; Rosenthal, Myron. / A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo. In: Analytical Biochemistry. 1985 ; Vol. 144, No. 2. pp. 483-493.
@article{6fd3d4bfba6a42e3af765096c5de56d2,
title = "A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo",
abstract = "Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.",
keywords = "cytochromes, mitochondrial redox state, optical methods, oxidative metabolism, rapid-scanning spectrophotometry, rat hippocampal slice",
author = "LaManna, {Joseph C.} and Pikarsky, {Solomon M.} and Thomas Sick and Myron Rosenthal",
year = "1985",
month = "2",
day = "1",
doi = "10.1016/0003-2697(85)90145-9",
language = "English",
volume = "144",
pages = "483--493",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo

AU - LaManna, Joseph C.

AU - Pikarsky, Solomon M.

AU - Sick, Thomas

AU - Rosenthal, Myron

PY - 1985/2/1

Y1 - 1985/2/1

N2 - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.

AB - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.

KW - cytochromes

KW - mitochondrial redox state

KW - optical methods

KW - oxidative metabolism

KW - rapid-scanning spectrophotometry

KW - rat hippocampal slice

UR - http://www.scopus.com/inward/record.url?scp=0022020794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022020794&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(85)90145-9

DO - 10.1016/0003-2697(85)90145-9

M3 - Article

C2 - 3993912

AN - SCOPUS:0022020794

VL - 144

SP - 483

EP - 493

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -