TY - JOUR
T1 - A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo
AU - LaManna, Joseph C.
AU - Pikarsky, Solomon M.
AU - Sick, Thomas J.
AU - Rosenthal, Myron
N1 - Funding Information:
We gratefully acknowledge the efforts of Joseph Lev-asseur to design and construct the mechanical stage for the optical detection system. We are also grateful to Alpha Therapeutics Inc. for providing the perfhioronated hydrocarbon solution (Fh~osol-DA) used in some experiments and to Stephen Milito and Emily Solow for expert technical assistance throughout these experiments. Details concerning the American Optical rapid-scanning spectrophotometer were kindly provided by Robert Leonard, A0 Scientific Instruments Division, Wamer-Lambert Technologies, Inc., Buffalo, New York. This work was supported in part by PHS Grants HR 34003, NS 14325, NS 17949, and NS 05820.
PY - 1985/2/1
Y1 - 1985/2/1
N2 - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.
AB - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.
KW - cytochromes
KW - mitochondrial redox state
KW - optical methods
KW - oxidative metabolism
KW - rapid-scanning spectrophotometry
KW - rat hippocampal slice
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U2 - 10.1016/0003-2697(85)90145-9
DO - 10.1016/0003-2697(85)90145-9
M3 - Article
C2 - 3993912
AN - SCOPUS:0022020794
VL - 144
SP - 483
EP - 493
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -