Abstract
Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 483-493 |
| Number of pages | 11 |
| Journal | Analytical Biochemistry |
| Volume | 144 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 1985 |
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Keywords
- cytochromes
- mitochondrial redox state
- optical methods
- oxidative metabolism
- rapid-scanning spectrophotometry
- rat hippocampal slice
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo. / LaManna, Joseph C.; Pikarsky, Solomon M.; Sick, Thomas; Rosenthal, Myron.
In: Analytical Biochemistry, Vol. 144, No. 2, 01.02.1985, p. 483-493.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo
AU - LaManna, Joseph C.
AU - Pikarsky, Solomon M.
AU - Sick, Thomas
AU - Rosenthal, Myron
PY - 1985/2/1
Y1 - 1985/2/1
N2 - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.
AB - Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.
KW - cytochromes
KW - mitochondrial redox state
KW - optical methods
KW - oxidative metabolism
KW - rapid-scanning spectrophotometry
KW - rat hippocampal slice
UR - http://www.scopus.com/inward/record.url?scp=0022020794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022020794&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90145-9
DO - 10.1016/0003-2697(85)90145-9
M3 - Article
C2 - 3993912
AN - SCOPUS:0022020794
VL - 144
SP - 483
EP - 493
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -