A rapid-scanning spectrophotometer designed for biological tissues in vitro or in vivo

Joseph C. LaManna, Solomon M. Pikarsky, Thomas J. Sick, Myron Rosenthal

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Rapid and sensitive detection of optical signals from biological materials has been very useful in studies of cell physiology and biochemistry. A rapid-scanning spectrophotometer is described here that has the following advantages: (i) it can be used in transmission or reflection modes, (ii) it can rapidly accumulate spectra and simultaneous kinetic data, (iii) it has high accuracy and sensitivity, and (iv) it can analyze and store large amounts of spectral information. Evaluations described here are aimed toward the measurement of reduction/oxidation shifts of mitochondrial cytochromes in tissues, but the flexibility of the optical components makes this spectrophotometer adaptable to the study of light absorption changes of intrinsic or extrinsic optically active molecules in a variety of light scattering preparations, tissues, and organs in vitro or in vivo.

Original languageEnglish (US)
Pages (from-to)483-493
Number of pages11
JournalAnalytical Biochemistry
Volume144
Issue number2
DOIs
StatePublished - Feb 1 1985

Keywords

  • cytochromes
  • mitochondrial redox state
  • optical methods
  • oxidative metabolism
  • rapid-scanning spectrophotometry
  • rat hippocampal slice

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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