Abstract
A method is described for the simultaneous determination of the rates of regional cerebral blood flow (rCBF) and regional cerebral glucose utilization (rCMRgl) in 6-7 mg brain samples dissected from multiple areas of interest. The method utilizes [131I]-iodoantipyrine ([131l]IAP) to measure rCBF by indicator fractionation, and [14C]2-deoxyglucose to measure rCMRgl. [131I]IAP was synthesized with specific activity exceeding 350 Ci/mmol and radiochemical purity greater than 99.5% by the radioiodination of antipyrine with Na131I. A triple-counting strategy was developed to quantitate 14C activity of the dissected brain samples in the presence of 131I. The factors contributing to the propagated error of the double-label separation strategy were defined and optimal assay parameters were determined. The separation strategy was validated by measuring rCBF simultaneously with both [131I]IAP (x) and [14C]IAP (y) in a series of rats. The equation of the regression line was y = 1.025 x -0.065 (correlation coefficient 0.985), denoting excellent agreement. In another series of 5 normocapnic rats anesthetized with nitrous oxide, rCBF and rCMRgl were measured simultaneously. In individual animals, the rates of rCBF within 14-16 brain areas were closely coupled to their respective rates of glucose metabolism. For the group data, the linear regression equation relating rCBF (y) to rCMRgl (x) was y = 1.76 x + 0.13 (correlation coefficient 0.93, P < 0.001). These studies provide direct evidence, based upon data obtained in the same brain, of a close coupling of regional metabolic rate and blood flow.
Original language | English (US) |
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Pages (from-to) | 165-179 |
Number of pages | 15 |
Journal | Brain research |
Volume | 230 |
Issue number | 1-2 |
DOIs | |
State | Published - Dec 28 1981 |
Keywords
- 2-deoxyglucose
- iodine
- double-label
- indicator fractionation
- iodoanipyrine
- nitrous oxide
- regional cerebral blood flow
- regional cerebral glucose utilization
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)