A radiation hybrid mapping panel for the rhesus macaque

William J. Murphy, J. E. Page, C. Smith, Ronald Charles Desrosiers, S. J. O'Brien

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The genomes of nonhuman primates have recently become highly visible candidates for full genome analysis, as they provide powerful models of human disease and a better understanding of the evolution of the human genome. We describe the creation of a 5000 rad radiation hybrid (RH) mapping panel for the rhesus macaque. Duplicate genotypes of 84 microsatellite and coding gene sequence tagged sites from six macaque chromosomes produced an estimated whole genome retention frequency of 0.33. To test the mapping ability of the panel, we constructed RH maps for macaque chromosomes 7 and 9 and compared them to orthologous locus orders in existing human and baboon maps derived from different methodologies. Concordant marker order between all three species maps suggests that the current panel represents a powerful mapping resource for generating high-density comparative maps of the rhesus macaque and other species genomes.

Original languageEnglish (US)
Pages (from-to)516-519
Number of pages4
JournalJournal of Heredity
Volume92
Issue number6
StatePublished - 2001
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)

Cite this

Murphy, W. J., Page, J. E., Smith, C., Desrosiers, R. C., & O'Brien, S. J. (2001). A radiation hybrid mapping panel for the rhesus macaque. Journal of Heredity, 92(6), 516-519.