A radiation hybrid breakpoint map of the acute myeloid leukemia (AML) and limb-girdle muscular dystrophy 1A (LGMD1A) regions of chromosome 5q31 localizing 122 expressed sequences

Stephen K. Horrigan, Lucia Bartoloni, Marcy C. Speer, Noreen Fulton, Jelena Kravarusic, Rajkumar Ramesar, Jeffery M Vance, Larry H. Yamaoka, Carol A. Westbrook

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have constructed a high-resolution map of a 6-Mb interval of human chromosome 5, band q31, incorporating 175 sequence tagged sites, of which 33 are genetic polymorphisms and 122 are nonredundant expressed sequences. The map was assembled initially as a YAC contig, incorporating data from radiation hybrid maps. To improve resolution and to identify errors in the databases, a radiation hybrid breakpoint map was developed for the interval, which included hybrids from both Stanford G3 and GeneBridge 4 panels. This novel approach facilitated the integration of one RH panel with another and enabled the identification and localization of new, previously unmapped ESTs from the radiation hybrid databases. ESTs were assembled into overlapping transcription units and ordered with respect to polymorphic markers in the region, resulting in a comprehensive map that incorporates markers from multiple different types of maps. This map of 5q31 will facilitate gene discovery efforts for several disorders, including limb-girdle muscular dystrophy type 1A and the genes deleted in acute myeloid leukemias and myelodysplasia. The study demonstrates the utility of a radiation hybrid breakpoint panel for correction of map errors and for the efficient identification of new transcript units in a large genom interval.

Original languageEnglish
Pages (from-to)24-35
Number of pages12
JournalGenomics
Volume57
Issue number1
DOIs
StatePublished - Apr 1 1999
Externally publishedYes

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Radiation Hybrid Mapping
Acute Myeloid Leukemia
Chromosomes
Expressed Sequence Tags
Sequence Tagged Sites
Databases
Chromosomes, Human, Pair 5
Genetic Association Studies
Human Chromosomes
Genetic Polymorphisms
Muscular dystrophy, limb-girdle, type 1A
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

A radiation hybrid breakpoint map of the acute myeloid leukemia (AML) and limb-girdle muscular dystrophy 1A (LGMD1A) regions of chromosome 5q31 localizing 122 expressed sequences. / Horrigan, Stephen K.; Bartoloni, Lucia; Speer, Marcy C.; Fulton, Noreen; Kravarusic, Jelena; Ramesar, Rajkumar; Vance, Jeffery M; Yamaoka, Larry H.; Westbrook, Carol A.

In: Genomics, Vol. 57, No. 1, 01.04.1999, p. 24-35.

Research output: Contribution to journalArticle

Horrigan, Stephen K. ; Bartoloni, Lucia ; Speer, Marcy C. ; Fulton, Noreen ; Kravarusic, Jelena ; Ramesar, Rajkumar ; Vance, Jeffery M ; Yamaoka, Larry H. ; Westbrook, Carol A. / A radiation hybrid breakpoint map of the acute myeloid leukemia (AML) and limb-girdle muscular dystrophy 1A (LGMD1A) regions of chromosome 5q31 localizing 122 expressed sequences. In: Genomics. 1999 ; Vol. 57, No. 1. pp. 24-35.
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