A Protocol for Characterizing the Impact of Collateral Flow after Distal Middle Cerebral Artery Occlusion

Richard Anthony DeFazio, Sean Levy, Carmen L. Morales, Rebecca V. Levy, Hung W. Lin, Tatjana Abaffy, Kunjan R. Dave, Brant D. Watson, Miguel A. Perez-Pinzon, Victoria Ohanna

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

In humans and in animal models of stroke, collateral blood flow between territories of the major pial arteries has a profound impact on cortical infarct size. However, there is a gap in our understanding of the genetic determinants of collateral formation and flow, as well as the signaling pathways and neurovascular interactions regulating this flow. Previous studies have demonstrated that collateral flow between branches of the anterior cerebral artery and the middle cerebral artery (MCA) can protect mouse cortex from infarction after MCA occlusion. Because the number and diameter of collaterals vary among mouse strains and after transgenic manipulations, a combination of methods is required to control for these variations. Here, we report an inexpensive approach to characterizing the cerebrovascular anatomy, and in vivo monitoring of cerebral blood flow as well. Further, we introduce a new, minimally invasive method for the occlusion of distal MCA branches. These methods will permit a new generation of studies on the mechanisms regulating collateral remodeling and cortical blood flow after stroke.

Original languageEnglish (US)
Pages (from-to)112-127
Number of pages16
JournalTranslational stroke research
Volume2
Issue number1
DOIs
StatePublished - Mar 2011

Keywords

  • Anastomoses
  • Collateral blood flow
  • Laser speckle imaging
  • Photothrombosis
  • Quantum dots
  • Vessel painting

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Cardiology and Cardiovascular Medicine

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