A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine

Kevin L. Schauer, Christophe M R Lemoine, Adrian Pelin, Nicolas Corradi, Wesley C. Warren, Martin Grosell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Marine teleost fish produce CaCO3 in their intestine as part of their osmoregulatory strategy. This precipitation is critical for rehydration and survival of the largest vertebrate group on earth, yet the molecular mechanisms that regulate this reaction are unknown. Here, we isolate and characterize an organic matrix associated with the intestinal precipitates produced by Gulf toadfish (Opsanus beta). Toadfish precipitates were purified using two different methods, and the associated organic matrix was extracted. Greater than 150 proteins were identified in the isolated matrix by mass spectrometry and subsequent database searching using an O. beta transcriptomic sequence library produced here. Many of the identified proteins were enriched in the matrix compared to the intestinal fluid, and three showed no substantial homology to any previously characterized protein in the NCBI database. To test the functionality of the isolated matrix, a micro-modified in vitro calcification assay was designed, which revealed that low concentrations of isolated matrix substantially promoted CaCO3 production, where high concentrations showed an inhibitory effect. High concentrations of matrix also decreased the incorporation of magnesium into the forming mineral, potentially providing an explanation for the variability in magnesium content observed in precipitates produced by different fish species.

Original languageEnglish (US)
Article number34494
JournalScientific Reports
Volume6
DOIs
StatePublished - Oct 3 2016

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Batrachoidiformes
Carbonates
Intestines
Minerals
Magnesium
Fishes
Databases
Proteins
Fluid Therapy
Libraries
Vertebrates
Mass Spectrometry

ASJC Scopus subject areas

  • General

Cite this

A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine. / Schauer, Kevin L.; Lemoine, Christophe M R; Pelin, Adrian; Corradi, Nicolas; Warren, Wesley C.; Grosell, Martin.

In: Scientific Reports, Vol. 6, 34494, 03.10.2016.

Research output: Contribution to journalArticle

Schauer, Kevin L. ; Lemoine, Christophe M R ; Pelin, Adrian ; Corradi, Nicolas ; Warren, Wesley C. ; Grosell, Martin. / A proteinaceous organic matrix regulates carbonate mineral production in the marine teleost intestine. In: Scientific Reports. 2016 ; Vol. 6.
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