Crude protein-containing surfactant, obtained from pig lungs, was centrifuged over 0.68 M sucrose at 5000 x g, 20°C. The floating pellicle was heated to 90°C under N2 and recentrifuged over sucrose. The pellicle was extracted with chloroform-methanol, dried and suspended in saline at 100 mg lipids/ml. This procedure results in a virtually protein-free, yet physiologically active lung surfactant. Minimal surface tension of the final preparation, evaluated with pulsating bubble at 37°C and 50% compression was 1.4 ± 1.4 mN/m. Tracheal instillation of 50 μl of this surfactant in premature newborn rabbits (day 27 of gestation) resulted in increased lung-thorax compliance (0.54 ± 0.09 vs. control level 0.33 ± 0.03 ml/cm H2O·kg; P < 0.002) and improved alveolar expansion. The surfactant preparation also offered effective prophylaxis against bronchiolar epithelial lesions during artificial ventilation.
|Original language||English (US)|
|Number of pages||2|
|Journal||IRCS Medical Science|
|State||Published - Jan 1 1981|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)