A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout

B. Baldisserotto, C. Kamunde, A. Matsuo, C. M. Wood

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The present study examined the interactions between elevated dietary calcium (as ionic Ca2+ in the form of CaCl2·2H 2O) and acute waterborne Cd exposure (50μg/l as CdNO3 for 3h) on whole body uptake and internal distribution of newly accumulated Cd, Ca2+, and Na+ in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed with three diets 20 (control), 30 and 60mg Ca 2+/g food: for 7 days before fluxes were measured with radiotracers over a 3h period. The two elevated Ca2+ diets reduced the whole body uptake of both Ca2+ and Cd by >50% and similarly reduced the internalization of both newly accumulated metals in most tissues, effects which reflect the shared branchial uptake route for Ca2+ and Cd. As the Ca2+ concentrations of the fluid phases of the stomach and intestinal contents were greatly elevated by the experimental diets, increased gastrointestinal Ca2+ uptake likely caused the down-regulation of the branchial Ca2+ (and Cd) uptake pathway. Waterborne Na + uptake and internal distribution were not affected. While plasma Ca2+ surged after the first two feedings of the 60mg Ca 2+/g diet, internal homeostasis was quickly restored. Total Ca 2+, Na+, and Cl- levels in tissues were not affected by diets. While dietary Ca2+ protected against waterborne Cd uptake, it did not protect against the relative inhibition of waterborne Ca2+ uptake caused by waterborne Cd. Acute exposure to 50μg/l Cd reduced the uptake and internalization of newly accumulated Ca2+ (but not Na+) by 70% or more, regardless of diet. Since elevated dietary Ca2+ reduces waterborne Cd uptake, fish eating a Ca 2+-rich invertebrate diet may be more protected against waterborne Cd toxicity in a field situation.

Original languageEnglish
Pages (from-to)57-73
Number of pages17
JournalAquatic Toxicology
Volume67
Issue number1
DOIs
StatePublished - Mar 30 2004

Fingerprint

Dietary Calcium
Oncorhynchus mykiss
Cadmium
rainbow
protective effect
cadmium
calcium
diet
Diet
uptake mechanisms
Gastrointestinal Contents
Fishes
homeostasis
Invertebrates
fish
effect
Homeostasis
Down-Regulation
Eating
invertebrate

Keywords

  • Cadmium uptake
  • Calcium uptake
  • Dietary calcium
  • Fish
  • Sodium uptake

ASJC Scopus subject areas

  • Aquatic Science

Cite this

A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout. / Baldisserotto, B.; Kamunde, C.; Matsuo, A.; Wood, C. M.

In: Aquatic Toxicology, Vol. 67, No. 1, 30.03.2004, p. 57-73.

Research output: Contribution to journalArticle

Baldisserotto, B. ; Kamunde, C. ; Matsuo, A. ; Wood, C. M. / A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout. In: Aquatic Toxicology. 2004 ; Vol. 67, No. 1. pp. 57-73.
@article{e4f5e9e11456489598fe73fd053ef7ee,
title = "A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout",
abstract = "The present study examined the interactions between elevated dietary calcium (as ionic Ca2+ in the form of CaCl2·2H 2O) and acute waterborne Cd exposure (50μg/l as CdNO3 for 3h) on whole body uptake and internal distribution of newly accumulated Cd, Ca2+, and Na+ in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed with three diets 20 (control), 30 and 60mg Ca 2+/g food: for 7 days before fluxes were measured with radiotracers over a 3h period. The two elevated Ca2+ diets reduced the whole body uptake of both Ca2+ and Cd by >50{\%} and similarly reduced the internalization of both newly accumulated metals in most tissues, effects which reflect the shared branchial uptake route for Ca2+ and Cd. As the Ca2+ concentrations of the fluid phases of the stomach and intestinal contents were greatly elevated by the experimental diets, increased gastrointestinal Ca2+ uptake likely caused the down-regulation of the branchial Ca2+ (and Cd) uptake pathway. Waterborne Na + uptake and internal distribution were not affected. While plasma Ca2+ surged after the first two feedings of the 60mg Ca 2+/g diet, internal homeostasis was quickly restored. Total Ca 2+, Na+, and Cl- levels in tissues were not affected by diets. While dietary Ca2+ protected against waterborne Cd uptake, it did not protect against the relative inhibition of waterborne Ca2+ uptake caused by waterborne Cd. Acute exposure to 50μg/l Cd reduced the uptake and internalization of newly accumulated Ca2+ (but not Na+) by 70{\%} or more, regardless of diet. Since elevated dietary Ca2+ reduces waterborne Cd uptake, fish eating a Ca 2+-rich invertebrate diet may be more protected against waterborne Cd toxicity in a field situation.",
keywords = "Cadmium uptake, Calcium uptake, Dietary calcium, Fish, Sodium uptake",
author = "B. Baldisserotto and C. Kamunde and A. Matsuo and Wood, {C. M.}",
year = "2004",
month = "3",
day = "30",
doi = "10.1016/j.aquatox.2003.12.004",
language = "English",
volume = "67",
pages = "57--73",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A protective effect of dietary calcium against acute waterborne cadmium uptake in rainbow trout

AU - Baldisserotto, B.

AU - Kamunde, C.

AU - Matsuo, A.

AU - Wood, C. M.

PY - 2004/3/30

Y1 - 2004/3/30

N2 - The present study examined the interactions between elevated dietary calcium (as ionic Ca2+ in the form of CaCl2·2H 2O) and acute waterborne Cd exposure (50μg/l as CdNO3 for 3h) on whole body uptake and internal distribution of newly accumulated Cd, Ca2+, and Na+ in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed with three diets 20 (control), 30 and 60mg Ca 2+/g food: for 7 days before fluxes were measured with radiotracers over a 3h period. The two elevated Ca2+ diets reduced the whole body uptake of both Ca2+ and Cd by >50% and similarly reduced the internalization of both newly accumulated metals in most tissues, effects which reflect the shared branchial uptake route for Ca2+ and Cd. As the Ca2+ concentrations of the fluid phases of the stomach and intestinal contents were greatly elevated by the experimental diets, increased gastrointestinal Ca2+ uptake likely caused the down-regulation of the branchial Ca2+ (and Cd) uptake pathway. Waterborne Na + uptake and internal distribution were not affected. While plasma Ca2+ surged after the first two feedings of the 60mg Ca 2+/g diet, internal homeostasis was quickly restored. Total Ca 2+, Na+, and Cl- levels in tissues were not affected by diets. While dietary Ca2+ protected against waterborne Cd uptake, it did not protect against the relative inhibition of waterborne Ca2+ uptake caused by waterborne Cd. Acute exposure to 50μg/l Cd reduced the uptake and internalization of newly accumulated Ca2+ (but not Na+) by 70% or more, regardless of diet. Since elevated dietary Ca2+ reduces waterborne Cd uptake, fish eating a Ca 2+-rich invertebrate diet may be more protected against waterborne Cd toxicity in a field situation.

AB - The present study examined the interactions between elevated dietary calcium (as ionic Ca2+ in the form of CaCl2·2H 2O) and acute waterborne Cd exposure (50μg/l as CdNO3 for 3h) on whole body uptake and internal distribution of newly accumulated Cd, Ca2+, and Na+ in juvenile rainbow trout (Oncorhynchus mykiss). Fish were fed with three diets 20 (control), 30 and 60mg Ca 2+/g food: for 7 days before fluxes were measured with radiotracers over a 3h period. The two elevated Ca2+ diets reduced the whole body uptake of both Ca2+ and Cd by >50% and similarly reduced the internalization of both newly accumulated metals in most tissues, effects which reflect the shared branchial uptake route for Ca2+ and Cd. As the Ca2+ concentrations of the fluid phases of the stomach and intestinal contents were greatly elevated by the experimental diets, increased gastrointestinal Ca2+ uptake likely caused the down-regulation of the branchial Ca2+ (and Cd) uptake pathway. Waterborne Na + uptake and internal distribution were not affected. While plasma Ca2+ surged after the first two feedings of the 60mg Ca 2+/g diet, internal homeostasis was quickly restored. Total Ca 2+, Na+, and Cl- levels in tissues were not affected by diets. While dietary Ca2+ protected against waterborne Cd uptake, it did not protect against the relative inhibition of waterborne Ca2+ uptake caused by waterborne Cd. Acute exposure to 50μg/l Cd reduced the uptake and internalization of newly accumulated Ca2+ (but not Na+) by 70% or more, regardless of diet. Since elevated dietary Ca2+ reduces waterborne Cd uptake, fish eating a Ca 2+-rich invertebrate diet may be more protected against waterborne Cd toxicity in a field situation.

KW - Cadmium uptake

KW - Calcium uptake

KW - Dietary calcium

KW - Fish

KW - Sodium uptake

UR - http://www.scopus.com/inward/record.url?scp=1442359995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1442359995&partnerID=8YFLogxK

U2 - 10.1016/j.aquatox.2003.12.004

DO - 10.1016/j.aquatox.2003.12.004

M3 - Article

VL - 67

SP - 57

EP - 73

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

IS - 1

ER -