A prognostic test to predict the risk of metastasis in uveal melanoma based on a 15-gene expression profile

Research output: Chapter in Book/Report/Conference proceedingChapter

62 Scopus citations

Abstract

Uveal (ocular) melanoma is an aggressive cancer that metastasizes in up to half of patients. Uveal melanoma spreads preferentially to the liver, and the metastatic disease is almost always fatal. There are no effective therapies for advanced metastatic disease, so the most promising strategy for improving survival is to detect metastasis at an earlier stage or to treat high-risk patients in an adjuvant setting. An accurate test for identifying high-risk patients would allow for such personalized management as well as for stratification of high-risk patients into clinical trials of adjuvant therapy. We developed a gene expression profile (GEP) that distinguishes between primary uveal melanomas that have a low metastatic risk (class 1 tumors) and those with a high metastatic risk (class 2 tumors). We migrated the GEP from a high-density microarray platform to a 15-gene, qPCR-based assay that is now performed in a College of American Pathologists (CAP)-accredited Clinical Laboratory Improvement Amendments (CLIA)-certified laboratory on a routine clinical basis on very small samples obtained by fine needle aspiration and on archival formalin-fixed specimens. We collaborated with several centers to show that our specimen collection protocol was easily learned and performed and that it allowed samples to be safely and reliably transported from distant locations with a very low failure rate. Finally, we showed in a multicenter, prospective study that our GEP assay is highly accurate for predicting which patients will develop metastatic disease, and it was significantly superior to the previous gold standard, chromosome 3 testing for monosomy 3. This is the only prognostic test in uveal melanoma ever to undergo such extensive validation, and it is currently being used in a commercial format under the trade name DecisionDx-UM in over 100 centers in the USA and Canada.

Original languageEnglish (US)
Title of host publicationMolecular Diagnostics for Melanoma
Subtitle of host publicationMethods and Protocols
EditorsMagdalena Thurin, Francesco M. Marincola
Pages427-440
Number of pages14
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1102
ISSN (Print)1064-3745

Keywords

  • Gene expression profiling
  • Machine learning algorithm
  • Metastasis
  • Prognosis
  • Support vector machine
  • Uveal melanoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'A prognostic test to predict the risk of metastasis in uveal melanoma based on a 15-gene expression profile'. Together they form a unique fingerprint.

  • Cite this

    Harbour, J. W. (2014). A prognostic test to predict the risk of metastasis in uveal melanoma based on a 15-gene expression profile. In M. Thurin, & F. M. Marincola (Eds.), Molecular Diagnostics for Melanoma: Methods and Protocols (pp. 427-440). (Methods in Molecular Biology; Vol. 1102). https://doi.org/10.1007/978-1-62703-727-3_22