A procedure for RT-PCR amplification of mRNAs on histological specimens

H. Staecker, M. Cammer, R. Rubinstein, Thomas R Van De Water

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Detection in combination with localization of low copy gene expression can be difficult to achieve. The use of reverse transcription PCR on tissue sections with a fluorescent marker provides localization of mRNA expression on a cellular level, and when combined with confocal microscopy and image analysis it also allows for an estimate of the relative intensity of fluorescence. A good example of the application of this method is the localization of nerve growth factor (NGF) mRNA expression in the inner ear. NGF through indirect tests appears to be present in this system, yet NGF mRNA could not be localized with in situ hybridization using radiolabeled riboprobes. Using fluorescent in situ RT-PCR, we can easily detect the presence of NGF mRNA and localize it to specific cell types within the maturing inner ear.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalBioTechniques
Volume16
Issue number1
StatePublished - Jan 1 1994
Externally publishedYes

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Nerve Growth Factor
Amplification
Polymerase Chain Reaction
Messenger RNA
Inner Ear
Confocal microscopy
Transcription
Gene expression
Confocal Microscopy
Image analysis
Reverse Transcription
In Situ Hybridization
Fluorescence
Tissue
Gene Expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Staecker, H., Cammer, M., Rubinstein, R., & Van De Water, T. R. (1994). A procedure for RT-PCR amplification of mRNAs on histological specimens. BioTechniques, 16(1), 76-80.

A procedure for RT-PCR amplification of mRNAs on histological specimens. / Staecker, H.; Cammer, M.; Rubinstein, R.; Van De Water, Thomas R.

In: BioTechniques, Vol. 16, No. 1, 01.01.1994, p. 76-80.

Research output: Contribution to journalArticle

Staecker, H, Cammer, M, Rubinstein, R & Van De Water, TR 1994, 'A procedure for RT-PCR amplification of mRNAs on histological specimens', BioTechniques, vol. 16, no. 1, pp. 76-80.
Staecker H, Cammer M, Rubinstein R, Van De Water TR. A procedure for RT-PCR amplification of mRNAs on histological specimens. BioTechniques. 1994 Jan 1;16(1):76-80.
Staecker, H. ; Cammer, M. ; Rubinstein, R. ; Van De Water, Thomas R. / A procedure for RT-PCR amplification of mRNAs on histological specimens. In: BioTechniques. 1994 ; Vol. 16, No. 1. pp. 76-80.
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