A preliminary investigation into the extent of increased radioresistance or hyper-radiosensitivity in cells of hamster cell lines known to be deficient in DNA repair

K. Skov, Brian Marples, J. B. Matthews, M. C. Joiner, H. Zhou

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The response to low doses of X rays was assessed in cells of three hamster cell lines which are defective in DNA repair and was compared with their parental lines. Cells of the V79-derived double-strand break repair-deficient line XR-V15B showed no radioresistance in the 0.5-Gy range compared with the V79B wild type, but instead showed an exponential response. Cells of the single-strand break repair-deficient line EM9 showed hyper-radiosensitivity and exhibited increased radioresistance. Most interestingly, cells of the UV- 20 cell line appeared to respond exponentially, as a continuation of the hyper-radiosensitive portion of the curve, with no evidence of increased radioresistance. This line is defective in an incision step of excision repair and is sensitive to crosslinking agents. Further studies are warranted to address the possible role of single- and double-strand break repair and excision repair in hyper-radiosensitivity and increased radioresistance.

Original languageEnglish (US)
JournalRadiation Research
Volume138
Issue number1 SUPPL.
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

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DNA Repair-Deficiency Disorders
hamsters
Radiation Tolerance
radiation tolerance
cultured cells
Cricetinae
deoxyribonucleic acid
DNA Repair
strands
Cell Line
cells
crosslinking
X-Rays
dosage
curves
x rays

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

A preliminary investigation into the extent of increased radioresistance or hyper-radiosensitivity in cells of hamster cell lines known to be deficient in DNA repair. / Skov, K.; Marples, Brian; Matthews, J. B.; Joiner, M. C.; Zhou, H.

In: Radiation Research, Vol. 138, No. 1 SUPPL., 01.01.1994.

Research output: Contribution to journalArticle

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