A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls

Chi Zhang, Dipak K. Dube, Xupei Huang, Robert W. Zajdel, Rajula Bhatia, Dalton Foster, Sharon L. Lemanski, Larry F. Lemanski

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ambystoma mexicanum is an intriguing animal model for studying heart development because it carries a mutation in gene c. Hearts of homozygous recessive (c/c) mutant embryos do not contain organized myofibrils and fail to beat. The defect can be corrected by organ-culturing the mutant heart in the presence of RNA from anterior endoderm or endoderm/mesoderm-conditioned medium. By screening a cDNA library made of total conditioned medium RNA from normal axolotl embryonic endoderm, we isolated a single clone (MIR), the synthetic RNA from which corrects the mutant heart defect by promoting myofibrillogenesis and thus was named MIR (myofibrillogenesis inducing RNA). In the present study, we have examined MIR gene expression in mutant axolotl hearts at early pre-heart-beat developmental stages and found its quantitative expression, as detected by RT-PCR, to be the same as in normal hearts. However, careful analysis of sequence data revealed a G→U point mutation in the mutant MIR RNA. Further computational analyses, using GENEBEE software to compare normal and mutant MIR RNAs show a significant alteration in RNA secondary structure of the point-mutated MIR RNA. The results from bioassay and confocal microscopy immunofluorescent studies demonstrate that, unlike MIR RNA derived from normal embryos, the mutated MIR RNA does not promote myofibrillogenesis in mutant embryonic hearts and fails to rescue/correct the mutant heart defect.

Original languageEnglish
Pages (from-to)495-506
Number of pages12
JournalAnatomy and Embryology
Volume206
Issue number6
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Ambystoma mexicanum
Point Mutation
Heart Failure
RNA
Muscle Development
Endoderm
Conditioned Culture Medium
Embryonic Structures

Keywords

  • Ambystoma mexicanum
  • Gene c
  • Myofibrillogenesis
  • Organ-culturing
  • Secondary structure

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

Zhang, C., Dube, D. K., Huang, X., Zajdel, R. W., Bhatia, R., Foster, D., ... Lemanski, L. F. (2003). A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls. Anatomy and Embryology, 206(6), 495-506.

A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls. / Zhang, Chi; Dube, Dipak K.; Huang, Xupei; Zajdel, Robert W.; Bhatia, Rajula; Foster, Dalton; Lemanski, Sharon L.; Lemanski, Larry F.

In: Anatomy and Embryology, Vol. 206, No. 6, 01.05.2003, p. 495-506.

Research output: Contribution to journalArticle

Zhang, C, Dube, DK, Huang, X, Zajdel, RW, Bhatia, R, Foster, D, Lemanski, SL & Lemanski, LF 2003, 'A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls', Anatomy and Embryology, vol. 206, no. 6, pp. 495-506.
Zhang C, Dube DK, Huang X, Zajdel RW, Bhatia R, Foster D et al. A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls. Anatomy and Embryology. 2003 May 1;206(6):495-506.
Zhang, Chi ; Dube, Dipak K. ; Huang, Xupei ; Zajdel, Robert W. ; Bhatia, Rajula ; Foster, Dalton ; Lemanski, Sharon L. ; Lemanski, Larry F. / A point mutation in bioactive RNA results in the failure of mutant heart correction in mexican axolotls. In: Anatomy and Embryology. 2003 ; Vol. 206, No. 6. pp. 495-506.
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