Currently, no in vitro model of human superficial fungal infection exists that is routinely used for standardized testing of the antifungal efficacy of topically applied agents. The objective of this study was to examine the effects of topical antifungal and corticosteroid preparations using viable human epidermal/dermal composite skin cultures to establish an in vitro model of fungal infection. The composite human skin surface was inoculated with 4 mL of a homogeneous suspension of Trichophyton rubrum, Trichophyton mentogrophytes, Trichophyton tonsurans, Microsporum canis, or Candida albicans followed by application of topical antifungal or corticosteroid preparations. At specified intervals, the presence and extent of fungal infection were assessed histologically. Thriving dermatophyte and C albicans infections were detected within 3 days after inoculation. Antifungal activities of topically applied antifungal preparations were detected and quantified. Preparations containing corticosteroid alone failed to enhance T rubrum infection. Results of this study conclude that in vitro composite human skin cultures are capable of sustaining dermatophyte and C albicans infection and can be used to detect antifungal activity of antifungal preparations.
|Original language||English (US)|
|Number of pages||6|
|State||Published - Apr 1 2005|
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