A pilot study of bone marrow stromal cells intraocular transplantation in the S334 transgenic rats and Sprague-Dawley rats

Guanting Qiu, Shibo Tang, Liang Zhang, Rong Wen

Research output: Contribution to journalArticle

Abstract

PURPOSE: To investigate the survival and differentiation of human bone marrow stromal cells (BMSC) intraocular transplantation in newborn S334 retinal degeneration transgenic rats and (Sprague-Dawley)SD rats. METHODS: Human bone marrow stromal cells line was grown on the adhesive substrate in the condition media including a-Modified Eagle medium (a-MEM)/10% fetal bovine serum. The experiments were divided into four groups: Group 1: BMSC + (Retinoid Acid) RA transplanted in S334 transgenic rats (n = 5); Group 2: BMSC transplanted in S334 transgenic rats (n = 5); Group 3: BMSC + RA transplanted in SD rats (n = 5); Group 4: BMSC transplanted in SD rats (n = 5). 2 microl cell suspension (about 4 x 10(4) cells) was injected into the vitreous space in the transgenic rats and normal SD rats at Postnatal 1 (P1) respectively. The right eyes were treated eyes and the left eyes were used as control. At P14 and P23, the rats were killed and enucleated for histological assays using plastic section. RESULTS: In Group 1, the transplanted cells were well survived. They could continue to differentiate and participate in late-stage retinal development. The number of inner nuclear layer increased. Moreover, the host retina increased their thickness, but photoreceptor cells were not rescued from transplant. In Group 2, at P14, the BMSC continue to differentiate toward their linage cell fate and formed into hemorrhage island structures with few neurons if RA was not applied. Group 3, BMSC could survive, migrate. The number of inner nuclear layer increased also. In Group 4, it revealed that host retina structures were disorganized and transplant cells formed atypical proliferating mass. CONCLUSIONS: This pilot experiment indicated that bone marrow stromal cells could survival, differentiate and participate in the retinal development after transplanted into vitreous space in the new born transgenic rats and SD rats. Histological assays showed that transplanted cells integrated with inner nuclear layer of host retina. Thus, bone marrow stromal cells may be a useful vehicle for auto- transplantation for the therapy of variety of retinal degenerative disorders.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalYan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
Volume18
Issue number2
StatePublished - Jun 1 2002
Externally publishedYes

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Transgenic Rats
Cell Transplantation
Mesenchymal Stromal Cells
Sprague Dawley Rats
Retina
Transplants
Photoreceptor Cells
Retinal Degeneration
Eagles
Retinoids
Islands
Adhesives
Plastics
Cell Survival
Suspensions
Transplantation
Hemorrhage

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

A pilot study of bone marrow stromal cells intraocular transplantation in the S334 transgenic rats and Sprague-Dawley rats. / Qiu, Guanting; Tang, Shibo; Zhang, Liang; Wen, Rong.

In: Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu, Vol. 18, No. 2, 01.06.2002, p. 110-114.

Research output: Contribution to journalArticle

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abstract = "PURPOSE: To investigate the survival and differentiation of human bone marrow stromal cells (BMSC) intraocular transplantation in newborn S334 retinal degeneration transgenic rats and (Sprague-Dawley)SD rats. METHODS: Human bone marrow stromal cells line was grown on the adhesive substrate in the condition media including a-Modified Eagle medium (a-MEM)/10{\%} fetal bovine serum. The experiments were divided into four groups: Group 1: BMSC + (Retinoid Acid) RA transplanted in S334 transgenic rats (n = 5); Group 2: BMSC transplanted in S334 transgenic rats (n = 5); Group 3: BMSC + RA transplanted in SD rats (n = 5); Group 4: BMSC transplanted in SD rats (n = 5). 2 microl cell suspension (about 4 x 10(4) cells) was injected into the vitreous space in the transgenic rats and normal SD rats at Postnatal 1 (P1) respectively. The right eyes were treated eyes and the left eyes were used as control. At P14 and P23, the rats were killed and enucleated for histological assays using plastic section. RESULTS: In Group 1, the transplanted cells were well survived. They could continue to differentiate and participate in late-stage retinal development. The number of inner nuclear layer increased. Moreover, the host retina increased their thickness, but photoreceptor cells were not rescued from transplant. In Group 2, at P14, the BMSC continue to differentiate toward their linage cell fate and formed into hemorrhage island structures with few neurons if RA was not applied. Group 3, BMSC could survive, migrate. The number of inner nuclear layer increased also. In Group 4, it revealed that host retina structures were disorganized and transplant cells formed atypical proliferating mass. CONCLUSIONS: This pilot experiment indicated that bone marrow stromal cells could survival, differentiate and participate in the retinal development after transplanted into vitreous space in the new born transgenic rats and SD rats. Histological assays showed that transplanted cells integrated with inner nuclear layer of host retina. Thus, bone marrow stromal cells may be a useful vehicle for auto- transplantation for the therapy of variety of retinal degenerative disorders.",
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N2 - PURPOSE: To investigate the survival and differentiation of human bone marrow stromal cells (BMSC) intraocular transplantation in newborn S334 retinal degeneration transgenic rats and (Sprague-Dawley)SD rats. METHODS: Human bone marrow stromal cells line was grown on the adhesive substrate in the condition media including a-Modified Eagle medium (a-MEM)/10% fetal bovine serum. The experiments were divided into four groups: Group 1: BMSC + (Retinoid Acid) RA transplanted in S334 transgenic rats (n = 5); Group 2: BMSC transplanted in S334 transgenic rats (n = 5); Group 3: BMSC + RA transplanted in SD rats (n = 5); Group 4: BMSC transplanted in SD rats (n = 5). 2 microl cell suspension (about 4 x 10(4) cells) was injected into the vitreous space in the transgenic rats and normal SD rats at Postnatal 1 (P1) respectively. The right eyes were treated eyes and the left eyes were used as control. At P14 and P23, the rats were killed and enucleated for histological assays using plastic section. RESULTS: In Group 1, the transplanted cells were well survived. They could continue to differentiate and participate in late-stage retinal development. The number of inner nuclear layer increased. Moreover, the host retina increased their thickness, but photoreceptor cells were not rescued from transplant. In Group 2, at P14, the BMSC continue to differentiate toward their linage cell fate and formed into hemorrhage island structures with few neurons if RA was not applied. Group 3, BMSC could survive, migrate. The number of inner nuclear layer increased also. In Group 4, it revealed that host retina structures were disorganized and transplant cells formed atypical proliferating mass. CONCLUSIONS: This pilot experiment indicated that bone marrow stromal cells could survival, differentiate and participate in the retinal development after transplanted into vitreous space in the new born transgenic rats and SD rats. Histological assays showed that transplanted cells integrated with inner nuclear layer of host retina. Thus, bone marrow stromal cells may be a useful vehicle for auto- transplantation for the therapy of variety of retinal degenerative disorders.

AB - PURPOSE: To investigate the survival and differentiation of human bone marrow stromal cells (BMSC) intraocular transplantation in newborn S334 retinal degeneration transgenic rats and (Sprague-Dawley)SD rats. METHODS: Human bone marrow stromal cells line was grown on the adhesive substrate in the condition media including a-Modified Eagle medium (a-MEM)/10% fetal bovine serum. The experiments were divided into four groups: Group 1: BMSC + (Retinoid Acid) RA transplanted in S334 transgenic rats (n = 5); Group 2: BMSC transplanted in S334 transgenic rats (n = 5); Group 3: BMSC + RA transplanted in SD rats (n = 5); Group 4: BMSC transplanted in SD rats (n = 5). 2 microl cell suspension (about 4 x 10(4) cells) was injected into the vitreous space in the transgenic rats and normal SD rats at Postnatal 1 (P1) respectively. The right eyes were treated eyes and the left eyes were used as control. At P14 and P23, the rats were killed and enucleated for histological assays using plastic section. RESULTS: In Group 1, the transplanted cells were well survived. They could continue to differentiate and participate in late-stage retinal development. The number of inner nuclear layer increased. Moreover, the host retina increased their thickness, but photoreceptor cells were not rescued from transplant. In Group 2, at P14, the BMSC continue to differentiate toward their linage cell fate and formed into hemorrhage island structures with few neurons if RA was not applied. Group 3, BMSC could survive, migrate. The number of inner nuclear layer increased also. In Group 4, it revealed that host retina structures were disorganized and transplant cells formed atypical proliferating mass. CONCLUSIONS: This pilot experiment indicated that bone marrow stromal cells could survival, differentiate and participate in the retinal development after transplanted into vitreous space in the new born transgenic rats and SD rats. Histological assays showed that transplanted cells integrated with inner nuclear layer of host retina. Thus, bone marrow stromal cells may be a useful vehicle for auto- transplantation for the therapy of variety of retinal degenerative disorders.

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