Bacteriophage infection of E. coli cells deficient in the enzyme tRNA nucleotidyltransferase (cca mutants) resulted in greatly decreased production of viable progeny phage compared to wild type cells. This decrease amounted to as much as 90% in the case of T-even bacteriophages, and 50-65% for T-odd bacteriophages. However, infection by the RNA phages, Qβ and f2, was unaffected by the cca mutation. Examination of T4 infection of cca hosts of progeny particles were formed, but that most of these particles were nonviable. Possible functions for E. coli tRNA nucleotidyltransferase during bacteriophage infection are discussed.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and biophysical research communications|
|State||Published - Dec 20 1976|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology