Abstract
Conventional culture vessels are not designed for physiological oxygen (O2) delivery. Both hyperoxia and hypoxia-commonly observed when culturing cells in regular plasticware-have been linked to reduced cellular function and death. Pancreatic islets, used for the clinical treatment of diabetes, are especially sensitive to sub- and supraphysiological O2 concentrations. A result of current culture standards is that a high percentage of islet preparations are never transplanted because of cell death and loss of function in the 24-8 h postisolation. Here, we describe a new culture system designed to provide quasiphysiological oxygenation to islets in culture. The use of dishes where islets rest atop a perfluorocarbon (PFC)-based membrane, coupled with a careful adjustment of environmental O2 concentration to target the islet physiological p02 range, resulted in dramatic gains in viability and function. These observations underline the importance of approximating culture conditions as closely as possible to those of the native microenvironment, and fill a widely acknowledged gap in our ability to preserve islet functionality in vitro. As stem cell-derived insulin-producing cells are likely to suffer from the same limitations as those observed in real islets, our findings are especially timely in the context of current efforts to define renewable sources for transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 1723-1733 |
| Number of pages | 11 |
| Journal | Cell Transplantation |
| Volume | 22 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 11 2013 |
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Keywords
- Cell culture
- Cell viability
- Islets
- Oxygenation
- Perfluorocarbon (PFC)
ASJC Scopus subject areas
- Cell Biology
- Transplantation
- Biomedical Engineering
Cite this
A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function. / Fraker, Christopher; Cechin, Sirlene; Álvarez-Cubela, Silvia; Echeverri, Felipe; Bernal, Andrés; Poo, Ramon; Ricordi, Camillo; Inverardi, Luca A; Dominguez-Bendala, Juan.
In: Cell Transplantation, Vol. 22, No. 9, 11.09.2013, p. 1723-1733.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function
AU - Fraker, Christopher
AU - Cechin, Sirlene
AU - Álvarez-Cubela, Silvia
AU - Echeverri, Felipe
AU - Bernal, Andrés
AU - Poo, Ramon
AU - Ricordi, Camillo
AU - Inverardi, Luca A
AU - Dominguez-Bendala, Juan
PY - 2013/9/11
Y1 - 2013/9/11
N2 - Conventional culture vessels are not designed for physiological oxygen (O2) delivery. Both hyperoxia and hypoxia-commonly observed when culturing cells in regular plasticware-have been linked to reduced cellular function and death. Pancreatic islets, used for the clinical treatment of diabetes, are especially sensitive to sub- and supraphysiological O2 concentrations. A result of current culture standards is that a high percentage of islet preparations are never transplanted because of cell death and loss of function in the 24-8 h postisolation. Here, we describe a new culture system designed to provide quasiphysiological oxygenation to islets in culture. The use of dishes where islets rest atop a perfluorocarbon (PFC)-based membrane, coupled with a careful adjustment of environmental O2 concentration to target the islet physiological p02 range, resulted in dramatic gains in viability and function. These observations underline the importance of approximating culture conditions as closely as possible to those of the native microenvironment, and fill a widely acknowledged gap in our ability to preserve islet functionality in vitro. As stem cell-derived insulin-producing cells are likely to suffer from the same limitations as those observed in real islets, our findings are especially timely in the context of current efforts to define renewable sources for transplantation.
AB - Conventional culture vessels are not designed for physiological oxygen (O2) delivery. Both hyperoxia and hypoxia-commonly observed when culturing cells in regular plasticware-have been linked to reduced cellular function and death. Pancreatic islets, used for the clinical treatment of diabetes, are especially sensitive to sub- and supraphysiological O2 concentrations. A result of current culture standards is that a high percentage of islet preparations are never transplanted because of cell death and loss of function in the 24-8 h postisolation. Here, we describe a new culture system designed to provide quasiphysiological oxygenation to islets in culture. The use of dishes where islets rest atop a perfluorocarbon (PFC)-based membrane, coupled with a careful adjustment of environmental O2 concentration to target the islet physiological p02 range, resulted in dramatic gains in viability and function. These observations underline the importance of approximating culture conditions as closely as possible to those of the native microenvironment, and fill a widely acknowledged gap in our ability to preserve islet functionality in vitro. As stem cell-derived insulin-producing cells are likely to suffer from the same limitations as those observed in real islets, our findings are especially timely in the context of current efforts to define renewable sources for transplantation.
KW - Cell culture
KW - Cell viability
KW - Islets
KW - Oxygenation
KW - Perfluorocarbon (PFC)
UR - http://www.scopus.com/inward/record.url?scp=84883492705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883492705&partnerID=8YFLogxK
U2 - 10.3727/096368912X657873
DO - 10.3727/096368912X657873
M3 - Article
C2 - 23068091
AN - SCOPUS:84883492705
VL - 22
SP - 1723
EP - 1733
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 9
ER -