A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function

Christopher Fraker, Sirlene Cechin, Silvia Álvarez-Cubela, Felipe Echeverri, Andrés Bernal, Ramon Poo, Camillo Ricordi, Luca A Inverardi, Juan Dominguez-Bendala

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Conventional culture vessels are not designed for physiological oxygen (O2) delivery. Both hyperoxia and hypoxia-commonly observed when culturing cells in regular plasticware-have been linked to reduced cellular function and death. Pancreatic islets, used for the clinical treatment of diabetes, are especially sensitive to sub- and supraphysiological O2 concentrations. A result of current culture standards is that a high percentage of islet preparations are never transplanted because of cell death and loss of function in the 24-8 h postisolation. Here, we describe a new culture system designed to provide quasiphysiological oxygenation to islets in culture. The use of dishes where islets rest atop a perfluorocarbon (PFC)-based membrane, coupled with a careful adjustment of environmental O2 concentration to target the islet physiological p02 range, resulted in dramatic gains in viability and function. These observations underline the importance of approximating culture conditions as closely as possible to those of the native microenvironment, and fill a widely acknowledged gap in our ability to preserve islet functionality in vitro. As stem cell-derived insulin-producing cells are likely to suffer from the same limitations as those observed in real islets, our findings are especially timely in the context of current efforts to define renewable sources for transplantation.

Original languageEnglish
Pages (from-to)1723-1733
Number of pages11
JournalCell Transplantation
Volume22
Issue number9
DOIs
StatePublished - Sep 11 2013

Fingerprint

Fluorocarbons
Oxygenation
Islets of Langerhans
Cell Survival
Equipment and Supplies
Hyperoxia
Insulin
Cell death
Medical problems
Stem cells
Cell Death
Stem Cells
Transplantation
Oxygen
Membranes
In Vitro Techniques
Hypoxia

Keywords

  • Cell culture
  • Cell viability
  • Islets
  • Oxygenation
  • Perfluorocarbon (PFC)

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function. / Fraker, Christopher; Cechin, Sirlene; Álvarez-Cubela, Silvia; Echeverri, Felipe; Bernal, Andrés; Poo, Ramon; Ricordi, Camillo; Inverardi, Luca A; Dominguez-Bendala, Juan.

In: Cell Transplantation, Vol. 22, No. 9, 11.09.2013, p. 1723-1733.

Research output: Contribution to journalArticle

Fraker, Christopher ; Cechin, Sirlene ; Álvarez-Cubela, Silvia ; Echeverri, Felipe ; Bernal, Andrés ; Poo, Ramon ; Ricordi, Camillo ; Inverardi, Luca A ; Dominguez-Bendala, Juan. / A physiological pattern of oxygenation using perfluorocarbon-based culture devices maximizes pancreatic islet viability and enhances β-cell function. In: Cell Transplantation. 2013 ; Vol. 22, No. 9. pp. 1723-1733.
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