A phenotypically distinct subset of immature B cells exhibits partial activation, increased survival, and preferential expression of VhS107

Emily L. Wilson, Erin M. Sherwood, Anne M. King, Richard L. Riley

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We have observed that immature B cells (IgMlowIgD- in the bone marrow of adult BALB/c mice exhibit heterogeneity, with a distinct subpopulation (∼4-10%) expressing the CD43/S7 surface protein. These CD43/S7+ immature B cells often express other surface antigens associated with B cell activation (CD5, CD11b, PD-1). Generation of optimal numbers of CD43/S7+ immature B cells requires expression of a functional Btk protein, consistent with activation as a requisite for the CD43/S7+ immature B cell phenotype. Like typical CD43/S7- immature B cells, the CD43/S7+ immature B cells are predominantly resting cells, which are derived from cycling bone marrow B cell precursors. The CD43/S7+ immature B cell population exhibits enhanced survival in vivo upon administration of the apoptosis-inducing corticosteroid, dexamethasone. Finally, CD43/S7+ immature B cells show a fourfold increase in incidence of VhS107 μ heavy chain expression compared to the CD43/S7- immature B cells. Therefore, in adult murine bone marrow, the presence of a phenotypically distinct immature B cell population can be demonstrated which has undergone partial activation leading to increased survival and BCR-dependent Vh repertoire selection.

Original languageEnglish (US)
Pages (from-to)3398-3408
Number of pages11
JournalEuropean Journal of Immunology
Volume33
Issue number12
DOIs
StatePublished - Dec 2003

Keywords

  • Activation
  • Apoptosis
  • CD43
  • Immature B cells
  • Repertoire

ASJC Scopus subject areas

  • Immunology

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