A novel vesicle-associated protein (VAP-1)in sea urchin eggs containing multiple RNA-binding consensus sequences

N. R. Barton, E. M. Bonder, D. J. Fishkind, R. H. Warren, M. M. Pratt

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We have identified a novel high molecular weight, vesicle-associated protein (VAP-1) in the eggs of the sea urchin Strongylocentrotus purpuratus. Biochemical fractionation and immunofluorescence analysis of unfertilized eggs indicate that VAP-1 is a peripheral membrane protein associated with microsomal membrane fractions. Sequence analysis of partial VAP-1 cDNA clones reveals that the protein contains at least four RNA-binding consensus sequences. The RNA-binding sequences are separated by several glycine rich domains and this organization, RNA-binding domains separated by glycine rich sequences, is common to several RNA-binding proteins including the heterogeneous ribonuclear protein A1 and nucleolin. The characteristics of VAP-1 suggest that the protein may function as a multidomain RNA-binding protein. The possibility that VAP-1 may play a role in nuclear RNA processing is also discussed.

Original languageEnglish (US)
Pages (from-to)797-809
Number of pages13
JournalJournal of Cell Science
Volume103
Issue number3
StatePublished - Dec 1 1992

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Keywords

  • RNA-binding sequences
  • Sea urchin egg
  • Vesicles

ASJC Scopus subject areas

  • Cell Biology

Cite this

Barton, N. R., Bonder, E. M., Fishkind, D. J., Warren, R. H., & Pratt, M. M. (1992). A novel vesicle-associated protein (VAP-1)in sea urchin eggs containing multiple RNA-binding consensus sequences. Journal of Cell Science, 103(3), 797-809.