A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences

N. R. Barton, E. M. Bonder, D. J. Fishkind, R. H. Warren, M. M. Pratt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have identified a novel high molecular weight, vesicle-associated protein (VAP-1) in the eggs of the sea urchin Strongylocentrotus purpuratus. Biochemical fractionation and immunofluorescence analysis of unfertilized eggs indicate that VAP-1 is a peripheral membrane protein associated with microsomal membrane fractions. Sequence analysis of partial VAP-1 cDNA clones reveals that the protein contains at least four RNA-binding consensus sequences. The RNA-binding sequences are separated by several glycine rich domains and this organization, RNA-binding domains separated by glycine rich sequences, is common to several RNA-binding proteins including the heterogeneous ribonuclear protein A1 and nucleolin. The characteristics of VAP-1 suggest that the protein may function as a multidomain RNA-binding protein. The possibility that VAP-1 may play a role in nuclear RNA processing is also discussed.

Original languageEnglish
Pages (from-to)797-809
Number of pages13
JournalJournal of Cell Science
Volume103
Issue number3
StatePublished - Nov 1 1992
Externally publishedYes

Fingerprint

Consensus Sequence
Eggs
RNA
RNA-Binding Proteins
Proteins
Glycine
Strongylocentrotus purpuratus
Nuclear RNA
Sea Urchins
Fluorescent Antibody Technique
Ovum
Sequence Analysis
Membrane Proteins
Complementary DNA
Clone Cells
Molecular Weight
sea urchin vesicle-associated protein
human AOC3 protein
Membranes

Keywords

  • RNA-binding sequences
  • Sea urchin egg
  • Vesicles

ASJC Scopus subject areas

  • Cell Biology

Cite this

Barton, N. R., Bonder, E. M., Fishkind, D. J., Warren, R. H., & Pratt, M. M. (1992). A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences. Journal of Cell Science, 103(3), 797-809.

A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences. / Barton, N. R.; Bonder, E. M.; Fishkind, D. J.; Warren, R. H.; Pratt, M. M.

In: Journal of Cell Science, Vol. 103, No. 3, 01.11.1992, p. 797-809.

Research output: Contribution to journalArticle

Barton, NR, Bonder, EM, Fishkind, DJ, Warren, RH & Pratt, MM 1992, 'A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences', Journal of Cell Science, vol. 103, no. 3, pp. 797-809.
Barton, N. R. ; Bonder, E. M. ; Fishkind, D. J. ; Warren, R. H. ; Pratt, M. M. / A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences. In: Journal of Cell Science. 1992 ; Vol. 103, No. 3. pp. 797-809.
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