A novel use of centrifugal force for cell seeding into porous scaffolds

W. T. Godbey, B. S. Stacey Hindy, Matthew E. Sherman, Anthony Atala

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

A novel rotor was constructed to allow for the seeding of porous scaffolds via centrifugal force. Using cell seeding times of 10min, this method placed significantly (roughly 3-fold) more cells into poly(glycolic acid) scaffolds than 24h of spinner flask seeding or static seeding. There were no significant differences in the mitochondrial activity per cell between the 3 seeding methods. Cell distribution was noted to be homogeneous throughout the scaffold thickness for the centrifugation method, as opposed to surface seeding for the spinner flask method. Centrifugation was especially efficient at low cell concentrations (1.33×105cells/ml). This system is useful for the seeding of biomaterials having cylindrical or planar geometries, and may be used under conditions that require low cell numbers and/or short seeding time periods.

Original languageEnglish (US)
Pages (from-to)2799-2805
Number of pages7
JournalBiomaterials
Volume25
Issue number14
DOIs
StatePublished - Jun 2004

Keywords

  • Bioreactor
  • Cell Seeding
  • Centrifugation
  • Scaffold

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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    Godbey, W. T., Stacey Hindy, B. S., Sherman, M. E., & Atala, A. (2004). A novel use of centrifugal force for cell seeding into porous scaffolds. Biomaterials, 25(14), 2799-2805. https://doi.org/10.1016/j.biomaterials.2003.09.056