Abstract
A novel rotor was constructed to allow for the seeding of porous scaffolds via centrifugal force. Using cell seeding times of 10min, this method placed significantly (roughly 3-fold) more cells into poly(glycolic acid) scaffolds than 24h of spinner flask seeding or static seeding. There were no significant differences in the mitochondrial activity per cell between the 3 seeding methods. Cell distribution was noted to be homogeneous throughout the scaffold thickness for the centrifugation method, as opposed to surface seeding for the spinner flask method. Centrifugation was especially efficient at low cell concentrations (1.33×105cells/ml). This system is useful for the seeding of biomaterials having cylindrical or planar geometries, and may be used under conditions that require low cell numbers and/or short seeding time periods.
| Original language | English |
|---|---|
| Pages (from-to) | 2799-2805 |
| Number of pages | 7 |
| Journal | Biomaterials |
| Volume | 25 |
| Issue number | 14 |
| DOIs | |
| State | Published - Jun 1 2004 |
| Externally published | Yes |
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Keywords
- Bioreactor
- Cell Seeding
- Centrifugation
- Scaffold
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
A novel use of centrifugal force for cell seeding into porous scaffolds. / Godbey, W. T.; Stacey Hindy, B. S.; Sherman, Matthew E.; Atala, Anthony.
In: Biomaterials, Vol. 25, No. 14, 01.06.2004, p. 2799-2805.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel use of centrifugal force for cell seeding into porous scaffolds
AU - Godbey, W. T.
AU - Stacey Hindy, B. S.
AU - Sherman, Matthew E.
AU - Atala, Anthony
PY - 2004/6/1
Y1 - 2004/6/1
N2 - A novel rotor was constructed to allow for the seeding of porous scaffolds via centrifugal force. Using cell seeding times of 10min, this method placed significantly (roughly 3-fold) more cells into poly(glycolic acid) scaffolds than 24h of spinner flask seeding or static seeding. There were no significant differences in the mitochondrial activity per cell between the 3 seeding methods. Cell distribution was noted to be homogeneous throughout the scaffold thickness for the centrifugation method, as opposed to surface seeding for the spinner flask method. Centrifugation was especially efficient at low cell concentrations (1.33×105cells/ml). This system is useful for the seeding of biomaterials having cylindrical or planar geometries, and may be used under conditions that require low cell numbers and/or short seeding time periods.
AB - A novel rotor was constructed to allow for the seeding of porous scaffolds via centrifugal force. Using cell seeding times of 10min, this method placed significantly (roughly 3-fold) more cells into poly(glycolic acid) scaffolds than 24h of spinner flask seeding or static seeding. There were no significant differences in the mitochondrial activity per cell between the 3 seeding methods. Cell distribution was noted to be homogeneous throughout the scaffold thickness for the centrifugation method, as opposed to surface seeding for the spinner flask method. Centrifugation was especially efficient at low cell concentrations (1.33×105cells/ml). This system is useful for the seeding of biomaterials having cylindrical or planar geometries, and may be used under conditions that require low cell numbers and/or short seeding time periods.
KW - Bioreactor
KW - Cell Seeding
KW - Centrifugation
KW - Scaffold
UR - http://www.scopus.com/inward/record.url?scp=1042298980&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1042298980&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2003.09.056
DO - 10.1016/j.biomaterials.2003.09.056
M3 - Article
C2 - 14962558
AN - SCOPUS:1042298980
VL - 25
SP - 2799
EP - 2805
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 14
ER -