A novel translational regulation function for the simian virus 40 large-T antigen gene

Prithi Rajan, Sathyamangalam Swaminathan, Jiyue Zhu, Charles N. Cole, Glen Barber, Mary J. Tevethia, Bayar Thimmapaya

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Cells use the interferon-induced, double-stranded-RNA-dependent protein kinase PKR as a defense against virus infections. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2, resulting in the cessation of protein synthesis. Viruses have evolved various strategies to counteract this cellular defense. In this paper, we show that simian virus 40 (SV40) large-T antigen can antagonize the translational inhibitory effect resulting from the activation of PKR in virus-infected cells. Unlike the situation with other virus-host cell interactions, SV40 large-T antigen does not block the activation of PKR, suggesting that SV40 counteracts the cellular antiviral response mediated by PKR at a step downstream of PKR activation. Mutational analysis of large-T antigen indicates that a domain located between amino acids 400 and 600 of large-T antigen is responsible for this function. These results define a novel translational regulatory function for the SV40 large-T antigen.

Original languageEnglish (US)
Pages (from-to)785-795
Number of pages11
JournalJournal of virology
Volume69
Issue number2
DOIs
StatePublished - Feb 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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