A novel strategy for rapid and efficient isolation of human tumor-specific CD4+ and CD8+ T-cell clones

Seung Tae Lee, Shujuan Liu, Laszlo Radvanyi, Pariya Sukhumalchandra, Jeffrey J. Molldrem, Eric D. Wieder, Patrick Hwu, Yong Jun Liu, Larry W. Kwak, Gregory Lizée, Sattva S. Neelapu

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Adoptive therapy with antigen-specific T cells is a promising approach for the treatment of infectious diseases and cancer. However, cloning of antigen-specific T cells by the traditional approach of limiting dilution is a time-consuming, laborious, and inefficient process. Here, we describe a novel flow cytometric strategy for rapid isolation of human tumor antigen-specific T-cell clones by using T-cell receptor (TCR) Vβ antibodies in combination with carboxyfluorescein succinimidyl ester (CFSE)-based proliferation assay. The CFSE dilution following antigen stimulation identified proliferating antigen-specific T cells, and the TCRVβ antibodies allowed distinguishing T cells at the clonal level from a heterogeneous T-cell population. This method of TCRVβ/CFSE dilution was used for the isolation of four different human lymphoma and melanoma-specific CD4+ and CD8+ T-cell clones reactive against defined and undefined tumor antigens. Isolated tumor-specific T-cell clones could be expanded to large numbers ex vivo while maintaining phenotype, function, and tumor antigen specificity. The method was simple, efficient, and reproducible, and may have potential application for the development of adoptive immunotherapeutic strategies.

Original languageEnglish (US)
Pages (from-to)13-26
Number of pages14
JournalJournal of Immunological Methods
Volume331
Issue number1-2
DOIs
StatePublished - Feb 29 2008
Externally publishedYes

Keywords

  • Cloning
  • Human
  • T cells
  • TCR
  • Tumor
  • Vbeta

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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    Lee, S. T., Liu, S., Radvanyi, L., Sukhumalchandra, P., Molldrem, J. J., Wieder, E. D., Hwu, P., Liu, Y. J., Kwak, L. W., Lizée, G., & Neelapu, S. S. (2008). A novel strategy for rapid and efficient isolation of human tumor-specific CD4+ and CD8+ T-cell clones. Journal of Immunological Methods, 331(1-2), 13-26. https://doi.org/10.1016/j.jim.2007.09.006