A novel stem cell tag-less sorting method

B. Roda, G. Lanzoni, F. Alviano, A. Zattoni, R. Costa, A. Di Carlo, C. Marchionni, M. Franchina, F. Ricci, P. Tazzari, P. Pagliaro, S. Z. Scalinci, L. Bonsi, P. Reschiglian, G. P. Bagnara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The lack of homogeneity in stem cell preparation blurs standardization, which is recommended for successful applications. Since common procedures for stem cell sorting involve approaches which make use of fluorescent or magnetic tags which can be not precisely known or can affect cell functionality and recovery, novel, fast and noninvasive methods for cell sorting are sought. Among stem cells, mesenchymal stem cells (MSCs) are promising candidates for cell therapy applications. This work presents a fractionation protocol based on a proprietary technology for the tag-less, flow-assisted fractionation of MSCs. The process is based on differences in cell biophysical features and it guarantees minimal manipulation and maintenance of cell features. It can be applied to isolate and separate MSCs from potential phenotypically different contaminants; to distinguish MSCs derived from different sources, and to sort stem cells from an MSC population isolated from a single source to improve the differentiation yield.

Original languageEnglish (US)
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages195-198
Number of pages4
StatePublished - 2010
Externally publishedYes
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Publication series

NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume3

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/TerritoryUnited States
CityAnaheim, CA
Period6/21/106/24/10

Keywords

  • Cell fractionation
  • Flow-assisted cell separation
  • Mesenchymal stem cell
  • Stem cell
  • Tag-less cell sorting

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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