A novel program to design siRNAs simultaneously effective to highly variable virus genomes

Hui Sun Lee, Jeonghyun Ahn, Eun Jung Jun, Sanghwa Yang, Chul Hyun Joo, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A major concern of antiviral therapy using small interfering RNAs (siRNAs) targeting RNA viral genome is high sequence diversity and mutation rate due to genetic instability. To overcome this problem, it is indispensable to design siRNAs targeting highly conserved regions. We thus designed CAPSID (Convenient Application Program for siRNA Design), a novel bioinformatics program to identify siRNAs targeting highly conserved regions within RNA viral genomes. From a set of input RNAs of diverse sequences, CAPSID rapidly searches conserved patterns and suggests highly potent siRNA candidates in a hierarchical manner. To validate the usefulness of this novel program, we investigated the antiviral potency of universal siRNA for various Human enterovirus B (HEB) serotypes. Assessment of antiviral efficacy using Hela cells, clearly demonstrates that HEB-specific siRNAs exhibit protective effects against all HEBs examined. These findings strongly indicate that CAPSID can be applied to select universal antiviral siRNAs against highly divergent viral genomes.

Original languageEnglish (US)
Pages (from-to)431-435
Number of pages5
JournalBiochemical and biophysical research communications
Volume384
Issue number4
DOIs
StatePublished - Jul 10 2009
Externally publishedYes

Keywords

  • Antiviral activity
  • CAPSID
  • Enterovirus
  • Genetic instability
  • Sequence diversity
  • Small-interfering RNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A novel program to design siRNAs simultaneously effective to highly variable virus genomes'. Together they form a unique fingerprint.

Cite this