A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine

Marc Birkhahn, Anirban P. Mitra, Anthony J. Williams, Nancy J. Barr, Eila C. Skinner, John P. Stein, Donald G. Skinner, Yu Chong Tai, Ram Datar, Richard J Cote

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background Sensitivity of standard urine cytology for detecting urothelial carcinoma of the bladder (UCB) is low, attributable largely to its inability to process entire samples, paucicellularity and presence of background cells. Objective Evaluate performance and practical applicability of a novel portable microfiltration device for capture, enumeration and characterisation of exfoliated tumour cells in urine, and compare it with standard urine cytology for UCB detection. Methods A total of 54 urine and bladder wash samples from patients undergoing surveillance for UCB were prospectively evaluated by standard and microfilter-based urine cytology. Head-to-head comparison of quality and performance metrics, and cost effectiveness was conducted for both methodologies. Results Five samples were paucicellular by standard cytology; no samples processed by microfilter cytology were paucicellular. Standard cytology had 33.3% more samples with background cells that limited evaluation (p < 0.001). Microfilter cytology was more concordant (κ = 50.4%) than standard cytology (κ = 33.5%) with true UCB diagnosis. Sensitivity, specificity and accuracy were higher for microfilter cytology compared to standard cytology (53.3%/100%/79.2% versus 40%/95.8%/69.9%, respectively). Microfilter-captured cells were amenable to downstream on-chip molecular analyses. A 40 ml sample was processed in under 4 min by microfilter cytology compared to 5.5 min by standard cytology. Median microfilter cytology processing and set-up costs were approximately 63% cheaper and 80 times lower than standard cytology, respectively. Conclusions The microfiltration device represents a novel non-invasive UCB detection system that is economical, rapid, versatile and has potentially better quality and performance metrics than routine urine cytology, the current standard-of-care.

Original languageEnglish
Pages (from-to)3159-3168
Number of pages10
JournalEuropean Journal of Cancer
Volume49
Issue number15
DOIs
StatePublished - Oct 1 2013

Fingerprint

Urinary Bladder Neoplasms
Cell Biology
Urine
Equipment and Supplies
Urinary Bladder
Carcinoma
Standard of Care
Cost-Benefit Analysis

Keywords

  • Bladder cancer
  • Nanotechnology
  • Screening
  • Surveillance
  • Urine cytology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine. / Birkhahn, Marc; Mitra, Anirban P.; Williams, Anthony J.; Barr, Nancy J.; Skinner, Eila C.; Stein, John P.; Skinner, Donald G.; Tai, Yu Chong; Datar, Ram; Cote, Richard J.

In: European Journal of Cancer, Vol. 49, No. 15, 01.10.2013, p. 3159-3168.

Research output: Contribution to journalArticle

Birkhahn, M, Mitra, AP, Williams, AJ, Barr, NJ, Skinner, EC, Stein, JP, Skinner, DG, Tai, YC, Datar, R & Cote, RJ 2013, 'A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine', European Journal of Cancer, vol. 49, no. 15, pp. 3159-3168. https://doi.org/10.1016/j.ejca.2013.04.033
Birkhahn, Marc ; Mitra, Anirban P. ; Williams, Anthony J. ; Barr, Nancy J. ; Skinner, Eila C. ; Stein, John P. ; Skinner, Donald G. ; Tai, Yu Chong ; Datar, Ram ; Cote, Richard J. / A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine. In: European Journal of Cancer. 2013 ; Vol. 49, No. 15. pp. 3159-3168.
@article{597ff8bad6b04da7932abe3c8568e27b,
title = "A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine",
abstract = "Background Sensitivity of standard urine cytology for detecting urothelial carcinoma of the bladder (UCB) is low, attributable largely to its inability to process entire samples, paucicellularity and presence of background cells. Objective Evaluate performance and practical applicability of a novel portable microfiltration device for capture, enumeration and characterisation of exfoliated tumour cells in urine, and compare it with standard urine cytology for UCB detection. Methods A total of 54 urine and bladder wash samples from patients undergoing surveillance for UCB were prospectively evaluated by standard and microfilter-based urine cytology. Head-to-head comparison of quality and performance metrics, and cost effectiveness was conducted for both methodologies. Results Five samples were paucicellular by standard cytology; no samples processed by microfilter cytology were paucicellular. Standard cytology had 33.3{\%} more samples with background cells that limited evaluation (p < 0.001). Microfilter cytology was more concordant (κ = 50.4{\%}) than standard cytology (κ = 33.5{\%}) with true UCB diagnosis. Sensitivity, specificity and accuracy were higher for microfilter cytology compared to standard cytology (53.3{\%}/100{\%}/79.2{\%} versus 40{\%}/95.8{\%}/69.9{\%}, respectively). Microfilter-captured cells were amenable to downstream on-chip molecular analyses. A 40 ml sample was processed in under 4 min by microfilter cytology compared to 5.5 min by standard cytology. Median microfilter cytology processing and set-up costs were approximately 63{\%} cheaper and 80 times lower than standard cytology, respectively. Conclusions The microfiltration device represents a novel non-invasive UCB detection system that is economical, rapid, versatile and has potentially better quality and performance metrics than routine urine cytology, the current standard-of-care.",
keywords = "Bladder cancer, Nanotechnology, Screening, Surveillance, Urine cytology",
author = "Marc Birkhahn and Mitra, {Anirban P.} and Williams, {Anthony J.} and Barr, {Nancy J.} and Skinner, {Eila C.} and Stein, {John P.} and Skinner, {Donald G.} and Tai, {Yu Chong} and Ram Datar and Cote, {Richard J}",
year = "2013",
month = "10",
day = "1",
doi = "10.1016/j.ejca.2013.04.033",
language = "English",
volume = "49",
pages = "3159--3168",
journal = "European Journal of Cancer",
issn = "0959-8049",
publisher = "Elsevier Limited",
number = "15",

}

TY - JOUR

T1 - A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine

AU - Birkhahn, Marc

AU - Mitra, Anirban P.

AU - Williams, Anthony J.

AU - Barr, Nancy J.

AU - Skinner, Eila C.

AU - Stein, John P.

AU - Skinner, Donald G.

AU - Tai, Yu Chong

AU - Datar, Ram

AU - Cote, Richard J

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Background Sensitivity of standard urine cytology for detecting urothelial carcinoma of the bladder (UCB) is low, attributable largely to its inability to process entire samples, paucicellularity and presence of background cells. Objective Evaluate performance and practical applicability of a novel portable microfiltration device for capture, enumeration and characterisation of exfoliated tumour cells in urine, and compare it with standard urine cytology for UCB detection. Methods A total of 54 urine and bladder wash samples from patients undergoing surveillance for UCB were prospectively evaluated by standard and microfilter-based urine cytology. Head-to-head comparison of quality and performance metrics, and cost effectiveness was conducted for both methodologies. Results Five samples were paucicellular by standard cytology; no samples processed by microfilter cytology were paucicellular. Standard cytology had 33.3% more samples with background cells that limited evaluation (p < 0.001). Microfilter cytology was more concordant (κ = 50.4%) than standard cytology (κ = 33.5%) with true UCB diagnosis. Sensitivity, specificity and accuracy were higher for microfilter cytology compared to standard cytology (53.3%/100%/79.2% versus 40%/95.8%/69.9%, respectively). Microfilter-captured cells were amenable to downstream on-chip molecular analyses. A 40 ml sample was processed in under 4 min by microfilter cytology compared to 5.5 min by standard cytology. Median microfilter cytology processing and set-up costs were approximately 63% cheaper and 80 times lower than standard cytology, respectively. Conclusions The microfiltration device represents a novel non-invasive UCB detection system that is economical, rapid, versatile and has potentially better quality and performance metrics than routine urine cytology, the current standard-of-care.

AB - Background Sensitivity of standard urine cytology for detecting urothelial carcinoma of the bladder (UCB) is low, attributable largely to its inability to process entire samples, paucicellularity and presence of background cells. Objective Evaluate performance and practical applicability of a novel portable microfiltration device for capture, enumeration and characterisation of exfoliated tumour cells in urine, and compare it with standard urine cytology for UCB detection. Methods A total of 54 urine and bladder wash samples from patients undergoing surveillance for UCB were prospectively evaluated by standard and microfilter-based urine cytology. Head-to-head comparison of quality and performance metrics, and cost effectiveness was conducted for both methodologies. Results Five samples were paucicellular by standard cytology; no samples processed by microfilter cytology were paucicellular. Standard cytology had 33.3% more samples with background cells that limited evaluation (p < 0.001). Microfilter cytology was more concordant (κ = 50.4%) than standard cytology (κ = 33.5%) with true UCB diagnosis. Sensitivity, specificity and accuracy were higher for microfilter cytology compared to standard cytology (53.3%/100%/79.2% versus 40%/95.8%/69.9%, respectively). Microfilter-captured cells were amenable to downstream on-chip molecular analyses. A 40 ml sample was processed in under 4 min by microfilter cytology compared to 5.5 min by standard cytology. Median microfilter cytology processing and set-up costs were approximately 63% cheaper and 80 times lower than standard cytology, respectively. Conclusions The microfiltration device represents a novel non-invasive UCB detection system that is economical, rapid, versatile and has potentially better quality and performance metrics than routine urine cytology, the current standard-of-care.

KW - Bladder cancer

KW - Nanotechnology

KW - Screening

KW - Surveillance

KW - Urine cytology

UR - http://www.scopus.com/inward/record.url?scp=84884909772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884909772&partnerID=8YFLogxK

U2 - 10.1016/j.ejca.2013.04.033

DO - 10.1016/j.ejca.2013.04.033

M3 - Article

C2 - 23849827

AN - SCOPUS:84884909772

VL - 49

SP - 3159

EP - 3168

JO - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

IS - 15

ER -