A novel nucleolytic activity associated with rabbit liver tRNA nucleotidyltransferase

Murray P. Deutscher

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Purified preparations of rabbit liver tRNA nucleotidyltransferase contain a nucleolytic activity which removes terminal CMP residues from tRNA-C-C and tRNA-C-C-C. Other tRNA molecules, such as tRNA-C-C-A, tRNA-C-A, tRNA-C-U and tRNA-C are not substrates for this reaction. The activity exhibits a sharp optimum at about pH 10 and a divalent cation (Mg++ or Mn++) is required. The reaction is inhibited by ATP, CTP, pyrophosphate and potassium chloride. The relation of this activity to other reactions catalyzed by tRNA nucleotidyltransferase is discussed.

Original languageEnglish (US)
Pages (from-to)216-222
Number of pages7
JournalBiochemical and biophysical research communications
Volume52
Issue number1
DOIs
StatePublished - May 1 1973

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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