A novel nuclear export activity in HIV-1 matrix protein required for viral replication

Stefan Dupont, Natalla Sharova, Caryn DeHoratlus, Ching Man A Virbasius, Xiaochun Zhu, Alice G. Bukrinskaya, Mario Stevenson, Michael R. Green

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

An important aspect of the pathophysiology of human immunodeficiency virus type-1 (HIV-1) infection is the ability of the virus to replicate in non-dividing cells. HIV-1 matrix (MA), the amino-terminal domain of the Pr55 gag polyprotein (Pr55), bears a nuclear localization signal that promotes localization of the viral preintegration complex to the nucleus of non- dividing cells following virus entry. However, late during infection, MA, as part of Pr55, directs unspliced viral RNA to the plasma membrane, the site of virus assembly. How MA can mediate these two opposing targeting functions is not understood. Here we demonstrate that MA has a previously undescribed nuclear export activity. Although MA lacks the canonical leucine-rich nuclear export signal, nuclear export is mediated through the conserved Crmlp pathway and functions in both mammalian cells and yeast. A mutation that disrupts the MA nuclear export signal (MA-M4) mislocalizes Pr55 and genomic viral RNA to the nucleus, thereby severely impairing viral replication. Furthermore, we show that MA-M4 can act in a dominant-negative fashion to mislocalize genomic viral RNA even in the presence of wild-type MA. We conclude that the MA nuclear export signal is required to counteract the MA nuclear localization signal, thus ensuring the cytoplasmic availability of the components required for virion assembly.

Original languageEnglish
Pages (from-to)681-685
Number of pages5
JournalNature
Volume402
Issue number6762
DOIs
StatePublished - Dec 9 1999
Externally publishedYes

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Viral Matrix Proteins
Nuclear Export Signals
Cell Nucleus Active Transport
Viral RNA
HIV-1
Nuclear Localization Signals
gag Gene Products
Virus Assembly
Virus Internalization
Virus Diseases
Leucine
Virion
Yeasts
Cell Membrane
Viruses
Mutation
Infection

ASJC Scopus subject areas

  • General

Cite this

Dupont, S., Sharova, N., DeHoratlus, C., Virbasius, C. M. A., Zhu, X., Bukrinskaya, A. G., ... Green, M. R. (1999). A novel nuclear export activity in HIV-1 matrix protein required for viral replication. Nature, 402(6762), 681-685. https://doi.org/10.1038/45272

A novel nuclear export activity in HIV-1 matrix protein required for viral replication. / Dupont, Stefan; Sharova, Natalla; DeHoratlus, Caryn; Virbasius, Ching Man A; Zhu, Xiaochun; Bukrinskaya, Alice G.; Stevenson, Mario; Green, Michael R.

In: Nature, Vol. 402, No. 6762, 09.12.1999, p. 681-685.

Research output: Contribution to journalArticle

Dupont, S, Sharova, N, DeHoratlus, C, Virbasius, CMA, Zhu, X, Bukrinskaya, AG, Stevenson, M & Green, MR 1999, 'A novel nuclear export activity in HIV-1 matrix protein required for viral replication', Nature, vol. 402, no. 6762, pp. 681-685. https://doi.org/10.1038/45272
Dupont S, Sharova N, DeHoratlus C, Virbasius CMA, Zhu X, Bukrinskaya AG et al. A novel nuclear export activity in HIV-1 matrix protein required for viral replication. Nature. 1999 Dec 9;402(6762):681-685. https://doi.org/10.1038/45272
Dupont, Stefan ; Sharova, Natalla ; DeHoratlus, Caryn ; Virbasius, Ching Man A ; Zhu, Xiaochun ; Bukrinskaya, Alice G. ; Stevenson, Mario ; Green, Michael R. / A novel nuclear export activity in HIV-1 matrix protein required for viral replication. In: Nature. 1999 ; Vol. 402, No. 6762. pp. 681-685.
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