A novel myelin basic protein transcript variant in the murine central nervous system

Anddre Osmar Valdivia, Valentina Farr, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Myelin basic protein is a multifunctional protein whose primary role is to adhere membranes of the myelin sheath. There are various isoforms that have been identified, 6 distinct isoforms in human and 13 distinct isoforms in mice. These distinct isoforms are the product of alternative splicing of a single gene. The present study sought out to identify the different isoforms found in the murine central nervous system. Neuronal tissue (brain) from five different C57BL6/J mice at 2 months of age was harvested and used for mRNA extraction. mRNA was reversed transcribed to cDNA and transcripts were detected through PCR amplification and DNA agarose gel separation. Primers for exon 1, exon 5b and exon 11 of the myelin basic protein gene were used to capture all the possible transcripts that are naturally found in the murine central nervous system. Unknown transcript was sequenced at Genewiz facilities (South Plainfield, NJ) and mass spectrometry protein sequence analysis demonstrated the presence of a novel myelin basic protein transcript variant. We identified a novel transcript variant of myelin basic protein. This novel transcript variant corresponds to a myelin basic protein of 32.5 kDa which has not been previously reported. This novel transcript variant presents relevant clinical significance to various demyelinating diseases due to its contribution to the understanding of the natural state of the murine central nervous system.

Original languageEnglish (US)
JournalMolecular Biology Reports
DOIs
StatePublished - Jan 1 2019

Fingerprint

Myelin Basic Protein
Protein Isoforms
Central Nervous System
Exons
Messenger RNA
Protein Sequence Analysis
Alternative Splicing
Demyelinating Diseases
Myelin Sheath
Sepharose
Genes
Mass Spectrometry
Complementary DNA
Gels
Polymerase Chain Reaction
Membranes
DNA
Brain
Proteins

Keywords

  • Central nervous system
  • Golli myelin basic protein
  • Myelin basic protein
  • Myelin sheath
  • Transcript variant

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

A novel myelin basic protein transcript variant in the murine central nervous system. / Valdivia, Anddre Osmar; Farr, Valentina; Bhattacharya, Sanjoy K.

In: Molecular Biology Reports, 01.01.2019.

Research output: Contribution to journalArticle

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