A novel motif in HIV-1 Nef that regulates MIP-1β chemokine release in macrophages

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

All primate lentiviruses encode Nef, an accessory protein that is important for viral pathogenicity in vivo. Lentiviral Nef proteins regulate the release of chemokines (MIP-1 α/β) from infected macrophages, thereby enhancing virus dissemination (S. Swingler, A. Mann, J. Jacque, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, and M. Stevenson, Nat. Med. 5:997-1003, 1999). In the current study, we have identified a novel domain within Nef (K92EK) that is required for Nef-dependent MIP-1β production by infected macrophages. Mutations in this domain abrogated MIP-1β induction but did not affect other Nef-ascribed activities, such as CD4 or major histocompatibility complex (MHC) class I downregulation. This further underscores Nef as a modular protein with genetically separable activities that may contribute to its role in viral replication and pathogenicity.

Original languageEnglish
Pages (from-to)8327-8331
Number of pages5
JournalJournal of Virology
Volume84
Issue number16
DOIs
StatePublished - Aug 1 2010
Externally publishedYes

Fingerprint

nef Gene Products
chemokines
Human immunodeficiency virus 1
Chemokines
Virulence
HIV-1
macrophages
Primate Lentiviruses
Macrophages
pathogenicity
Major Histocompatibility Complex
Lentivirus
Down-Regulation
proteins
major histocompatibility complex
virus replication
Viruses
Mutation
Primates
mutation

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A novel motif in HIV-1 Nef that regulates MIP-1β chemokine release in macrophages. / Dai, Lue; Stevenson, Mario.

In: Journal of Virology, Vol. 84, No. 16, 01.08.2010, p. 8327-8331.

Research output: Contribution to journalArticle

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