Abstract
All primate lentiviruses encode Nef, an accessory protein that is important for viral pathogenicity in vivo. Lentiviral Nef proteins regulate the release of chemokines (MIP-1 α/β) from infected macrophages, thereby enhancing virus dissemination (S. Swingler, A. Mann, J. Jacque, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, and M. Stevenson, Nat. Med. 5:997-1003, 1999). In the current study, we have identified a novel domain within Nef (K92EK) that is required for Nef-dependent MIP-1β production by infected macrophages. Mutations in this domain abrogated MIP-1β induction but did not affect other Nef-ascribed activities, such as CD4 or major histocompatibility complex (MHC) class I downregulation. This further underscores Nef as a modular protein with genetically separable activities that may contribute to its role in viral replication and pathogenicity.
| Original language | English |
|---|---|
| Pages (from-to) | 8327-8331 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 84 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 1 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
A novel motif in HIV-1 Nef that regulates MIP-1β chemokine release in macrophages. / Dai, Lue; Stevenson, Mario.
In: Journal of Virology, Vol. 84, No. 16, 01.08.2010, p. 8327-8331.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel motif in HIV-1 Nef that regulates MIP-1β chemokine release in macrophages
AU - Dai, Lue
AU - Stevenson, Mario
PY - 2010/8/1
Y1 - 2010/8/1
N2 - All primate lentiviruses encode Nef, an accessory protein that is important for viral pathogenicity in vivo. Lentiviral Nef proteins regulate the release of chemokines (MIP-1 α/β) from infected macrophages, thereby enhancing virus dissemination (S. Swingler, A. Mann, J. Jacque, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, and M. Stevenson, Nat. Med. 5:997-1003, 1999). In the current study, we have identified a novel domain within Nef (K92EK) that is required for Nef-dependent MIP-1β production by infected macrophages. Mutations in this domain abrogated MIP-1β induction but did not affect other Nef-ascribed activities, such as CD4 or major histocompatibility complex (MHC) class I downregulation. This further underscores Nef as a modular protein with genetically separable activities that may contribute to its role in viral replication and pathogenicity.
AB - All primate lentiviruses encode Nef, an accessory protein that is important for viral pathogenicity in vivo. Lentiviral Nef proteins regulate the release of chemokines (MIP-1 α/β) from infected macrophages, thereby enhancing virus dissemination (S. Swingler, A. Mann, J. Jacque, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, and M. Stevenson, Nat. Med. 5:997-1003, 1999). In the current study, we have identified a novel domain within Nef (K92EK) that is required for Nef-dependent MIP-1β production by infected macrophages. Mutations in this domain abrogated MIP-1β induction but did not affect other Nef-ascribed activities, such as CD4 or major histocompatibility complex (MHC) class I downregulation. This further underscores Nef as a modular protein with genetically separable activities that may contribute to its role in viral replication and pathogenicity.
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U2 - 10.1128/JVI.00741-10
DO - 10.1128/JVI.00741-10
M3 - Article
C2 - 20504918
AN - SCOPUS:77954994071
VL - 84
SP - 8327
EP - 8331
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 16
ER -