A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors

K. Sollerbrant, J. Elmén, C. Wahlestedt, J. Acker, H. Leblois-Prehaud, M. Latta-Mahieu, P. Yeh, M. Perricaudet

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The baculovirus Autographa californica multiple nucleopolyhedrosis virus causes non-productive infection in mammalian cells. Recombinant baculovirus therefore has the capability to transfer and express heterologous genes in these cells if a mammalian promoter governs the gene of interest. We have investigated the possibility of using baculovirus as a tool to produce recombinant adeno-associated virus (rAAV). AAV has become increasingly popular as a vector for gene therapy and functional genomics efforts, although its use is hampered by the lack of a simple and efficient vector production method. We show here that co-infection of mammalian producer cells with three viruses - a baculovirus containing the reporter gene flanked by AAV ITRs, a baculovirus expressing the AAV rep gene and a helper adenovirus expressing the AAV cap gene - produces infectious rAAV particles. This baculovirus-based chimeric vector method may in future improve large-scale rAAV vector preparations and circumvent present-day problems associated with rAAV production.

Original languageEnglish (US)
Pages (from-to)2051-2060
Number of pages10
JournalJournal of General Virology
Volume82
Issue number9
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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    Sollerbrant, K., Elmén, J., Wahlestedt, C., Acker, J., Leblois-Prehaud, H., Latta-Mahieu, M., Yeh, P., & Perricaudet, M. (2001). A novel method using baculovirus-mediated gene transfer for production of recombinant adeno-associated virus vectors. Journal of General Virology, 82(9), 2051-2060. https://doi.org/10.1099/0022-1317-82-9-2051