A novel method of extraction of TnC from skeletal muscle myofibrils

L. D. Allhouse, J. D. Potter, C. C. Ashley

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Incubation of mechanically skinned barnacle myofibrillar bundles in 10 mM orthovanadate (pH 6.6) results in the loss of Ca2+-dependent force generation, which reduces to 0.98±0.006% (mean±SEM, n=25) of control levels. Analysis of myofibrillar bundles by gel electrophoresis showed that tension loss is primarily due to the extraction of troponin C (TnC) (65.4±5.04% mean ±SEM, n=5). This is a novel finding, since treating cardiac fibres with orthovanadate results in the removal of both TnC and troponin I (TnI) (28). Ca2+ dependence was restored to the myofibrillar bundles following reconstitution with either native isoform of barnacle TnC (BTnC1: 78.72±12.8%, n=9, BTnC2: 82.73±20.3%, n=3). The reversible loss of Ca2+-dependent tension generation following the removal and replacement of TnC indicates that the regulation of contraction in the barnacle is controlled by thin-filament regulatory proteins.

Original languageEnglish (US)
Pages (from-to)695-701
Number of pages7
JournalPflugers Archiv European Journal of Physiology
Volume437
Issue number5
DOIs
StatePublished - Apr 16 1999

Keywords

  • Calcium
  • Muscle
  • Skeletal
  • Vanadate

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'A novel method of extraction of TnC from skeletal muscle myofibrils'. Together they form a unique fingerprint.

  • Cite this