A novel method for the encapsulation of biomolecules into polymersomes via direct hydration

Conlin P. O'Neil, Tomoake Suzuki, Davide Demurtas, Andrija Finka, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present a novel method for encapsulation of proteins with high encapsulation efficiency within polymersomes formed from block copolymers of polyethylene glycol)-bl- poly(propylene sulfide). By formulation of the neat block copolymer with a low molecular weight polyethylene glycol), direct hydration of the formulated mixture yielded polymersomes. We were able to achieve encapsulation efficiencies for ovalbumin at 37%, bovine serum albumin at 19%, and bovine y-globulin at 15% when the proteins were included in the hydration solution. The formulation process and the dispersion of polymersomes from the preparation in phosphate-buffered saline were characterized using confocal microscopy, cryogenic transmission electron microscopy, and fluorimetry. We were also successful in the encapsulation of proteinase K, a proteolytic enzyme, and demonstrated by SDS-PAGE that the enzyme was contained inside polymersomes when dispersed in a solution of ovalbumin.

Original languageEnglish
Pages (from-to)9025-9029
Number of pages5
JournalLangmuir
Volume25
Issue number16
DOIs
StatePublished - Aug 18 2009
Externally publishedYes

Fingerprint

Biomolecules
block copolymers
Encapsulation
Hydration
hydration
enzymes
glycols
polyethylenes
Block copolymers
globulins
proteins
Ovalbumin
formulations
Polyethylene glycols
low molecular weights
albumins
polypropylene
serums
cryogenics
sulfides

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

O'Neil, C. P., Suzuki, T., Demurtas, D., Finka, A., & Hubbell, J. A. (2009). A novel method for the encapsulation of biomolecules into polymersomes via direct hydration. Langmuir, 25(16), 9025-9029. https://doi.org/10.1021/la900779t

A novel method for the encapsulation of biomolecules into polymersomes via direct hydration. / O'Neil, Conlin P.; Suzuki, Tomoake; Demurtas, Davide; Finka, Andrija; Hubbell, Jeffrey A.

In: Langmuir, Vol. 25, No. 16, 18.08.2009, p. 9025-9029.

Research output: Contribution to journalArticle

O'Neil, CP, Suzuki, T, Demurtas, D, Finka, A & Hubbell, JA 2009, 'A novel method for the encapsulation of biomolecules into polymersomes via direct hydration', Langmuir, vol. 25, no. 16, pp. 9025-9029. https://doi.org/10.1021/la900779t
O'Neil CP, Suzuki T, Demurtas D, Finka A, Hubbell JA. A novel method for the encapsulation of biomolecules into polymersomes via direct hydration. Langmuir. 2009 Aug 18;25(16):9025-9029. https://doi.org/10.1021/la900779t
O'Neil, Conlin P. ; Suzuki, Tomoake ; Demurtas, Davide ; Finka, Andrija ; Hubbell, Jeffrey A. / A novel method for the encapsulation of biomolecules into polymersomes via direct hydration. In: Langmuir. 2009 ; Vol. 25, No. 16. pp. 9025-9029.
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