Abstract
One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present a novel method for encapsulation of proteins with high encapsulation efficiency within polymersomes formed from block copolymers of polyethylene glycol)-bl- poly(propylene sulfide). By formulation of the neat block copolymer with a low molecular weight polyethylene glycol), direct hydration of the formulated mixture yielded polymersomes. We were able to achieve encapsulation efficiencies for ovalbumin at 37%, bovine serum albumin at 19%, and bovine y-globulin at 15% when the proteins were included in the hydration solution. The formulation process and the dispersion of polymersomes from the preparation in phosphate-buffered saline were characterized using confocal microscopy, cryogenic transmission electron microscopy, and fluorimetry. We were also successful in the encapsulation of proteinase K, a proteolytic enzyme, and demonstrated by SDS-PAGE that the enzyme was contained inside polymersomes when dispersed in a solution of ovalbumin.
| Original language | English |
|---|---|
| Pages (from-to) | 9025-9029 |
| Number of pages | 5 |
| Journal | Langmuir |
| Volume | 25 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 18 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Electrochemistry
- Condensed Matter Physics
- Surfaces and Interfaces
- Materials Science(all)
- Spectroscopy
Cite this
A novel method for the encapsulation of biomolecules into polymersomes via direct hydration. / O'Neil, Conlin P.; Suzuki, Tomoake; Demurtas, Davide; Finka, Andrija; Hubbell, Jeffrey A.
In: Langmuir, Vol. 25, No. 16, 18.08.2009, p. 9025-9029.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel method for the encapsulation of biomolecules into polymersomes via direct hydration
AU - O'Neil, Conlin P.
AU - Suzuki, Tomoake
AU - Demurtas, Davide
AU - Finka, Andrija
AU - Hubbell, Jeffrey A.
PY - 2009/8/18
Y1 - 2009/8/18
N2 - One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present a novel method for encapsulation of proteins with high encapsulation efficiency within polymersomes formed from block copolymers of polyethylene glycol)-bl- poly(propylene sulfide). By formulation of the neat block copolymer with a low molecular weight polyethylene glycol), direct hydration of the formulated mixture yielded polymersomes. We were able to achieve encapsulation efficiencies for ovalbumin at 37%, bovine serum albumin at 19%, and bovine y-globulin at 15% when the proteins were included in the hydration solution. The formulation process and the dispersion of polymersomes from the preparation in phosphate-buffered saline were characterized using confocal microscopy, cryogenic transmission electron microscopy, and fluorimetry. We were also successful in the encapsulation of proteinase K, a proteolytic enzyme, and demonstrated by SDS-PAGE that the enzyme was contained inside polymersomes when dispersed in a solution of ovalbumin.
AB - One of the major engineering challenges for the implementation of block copolymer vesicles, or polymersomes, as therapeutic drug carriers is obtaining high encapsulation efficiencies for biomolecules. Here we present a novel method for encapsulation of proteins with high encapsulation efficiency within polymersomes formed from block copolymers of polyethylene glycol)-bl- poly(propylene sulfide). By formulation of the neat block copolymer with a low molecular weight polyethylene glycol), direct hydration of the formulated mixture yielded polymersomes. We were able to achieve encapsulation efficiencies for ovalbumin at 37%, bovine serum albumin at 19%, and bovine y-globulin at 15% when the proteins were included in the hydration solution. The formulation process and the dispersion of polymersomes from the preparation in phosphate-buffered saline were characterized using confocal microscopy, cryogenic transmission electron microscopy, and fluorimetry. We were also successful in the encapsulation of proteinase K, a proteolytic enzyme, and demonstrated by SDS-PAGE that the enzyme was contained inside polymersomes when dispersed in a solution of ovalbumin.
UR - http://www.scopus.com/inward/record.url?scp=68649100989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68649100989&partnerID=8YFLogxK
U2 - 10.1021/la900779t
DO - 10.1021/la900779t
M3 - Article
C2 - 19621886
AN - SCOPUS:68649100989
VL - 25
SP - 9025
EP - 9029
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 16
ER -