A novel method for gene expression mapping of metastatic competence in human bladder cancer

Z. Wu, M. S. Siadaty, G. Riddick, H. F. Frierson, J. K. Lee, W. Golden, S. Knuutila, G. M. Hampton, Wael El-Rifai, D. Theodorescu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Expression profiling by DNA microarray analysis has provided insights into molecular alterations that underpin cancer progression and metastasis. Although differential expression of microarray-defined probes can be related to numerical or structural chromosomal alterations, it is unclear if such changes are also clustered in distinct chromosomes or genomic regions and whether chromosomal alterations always reflect changes in gene expression. Here we apply the dChip algorithm and a novel technique to test the hypothesis that expression changes occurring as a function of tumor progression and metastasis are nonrandomly distributed. Expression profiling of a human xenograft model of lung metastasis phenotype indicates that chromosomes 2, 11, and 20 contain higher percentages of differentially expressed genes ( P < .05). Furthermore, we show that a number of differentially expressed probes mapped to chromosome 17q, defining the existence of an expression "hot spot" corresponding to an area of gain determined by comparative genomic hybridization (CGH). Interestingly, other areas of gains detected by CGH were not associated with expression hot spots. In summary, we show that gene expression changes during bladder cancer lung metastasis occur nonrandomly in specific chromosomes and intrachromosomal locations.

Original languageEnglish (US)
Pages (from-to)181-189
Number of pages9
JournalNeoplasia
Volume8
Issue number3
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Fingerprint

Chromosome Mapping
Urinary Bladder Neoplasms
Mental Competency
Neoplasm Metastasis
Gene Expression
Comparative Genomic Hybridization
Chromosomes
Chromosomes, Human, Pair 20
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 2
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Heterografts
Lung Neoplasms
Neoplasms
Phenotype
Lung
Genes

Keywords

  • Bladder neoplasms
  • Comparative genomic hybridization
  • Gene expression
  • Karyotype
  • Metastasis

ASJC Scopus subject areas

  • Cancer Research

Cite this

Wu, Z., Siadaty, M. S., Riddick, G., Frierson, H. F., Lee, J. K., Golden, W., ... Theodorescu, D. (2006). A novel method for gene expression mapping of metastatic competence in human bladder cancer. Neoplasia, 8(3), 181-189. https://doi.org/10.1593/neo.05727

A novel method for gene expression mapping of metastatic competence in human bladder cancer. / Wu, Z.; Siadaty, M. S.; Riddick, G.; Frierson, H. F.; Lee, J. K.; Golden, W.; Knuutila, S.; Hampton, G. M.; El-Rifai, Wael; Theodorescu, D.

In: Neoplasia, Vol. 8, No. 3, 01.01.2006, p. 181-189.

Research output: Contribution to journalArticle

Wu, Z, Siadaty, MS, Riddick, G, Frierson, HF, Lee, JK, Golden, W, Knuutila, S, Hampton, GM, El-Rifai, W & Theodorescu, D 2006, 'A novel method for gene expression mapping of metastatic competence in human bladder cancer', Neoplasia, vol. 8, no. 3, pp. 181-189. https://doi.org/10.1593/neo.05727
Wu Z, Siadaty MS, Riddick G, Frierson HF, Lee JK, Golden W et al. A novel method for gene expression mapping of metastatic competence in human bladder cancer. Neoplasia. 2006 Jan 1;8(3):181-189. https://doi.org/10.1593/neo.05727
Wu, Z. ; Siadaty, M. S. ; Riddick, G. ; Frierson, H. F. ; Lee, J. K. ; Golden, W. ; Knuutila, S. ; Hampton, G. M. ; El-Rifai, Wael ; Theodorescu, D. / A novel method for gene expression mapping of metastatic competence in human bladder cancer. In: Neoplasia. 2006 ; Vol. 8, No. 3. pp. 181-189.
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