A novel K+ channel blocker isolated from 'hiccup nut' toxin

Ezia Guatteo, Laura Bianchi, Laura Faravelli, Luisella Verotta, Francesca Pelizzoni, Colin B. Rogers, Enzo Wanke

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Combretastatin B1, a polyhydroxybibenzyl compound extracted from the fruit of Combretum kraussii, known to contain 'hiccup nut' toxin, reversibly increased the duration, but not the peak or the rate of rise, of the action potential in rat sensory neurones by approximately 300%. This effect was only seen when it was applied to the extracellular side of the membrane. No effects on the resting potential were observed. K+ delayed rectifier currents were inhibited in neurones and in human myotubes with an IC50 of about 300 μM; the HERG-type inward rectifier channels in tumour cells were inhibited to a greater degree. Due to its selective action and the similarity of its blockade to that produced by class III antiarrhythmic drugs, the toxin could be the origin of compounds of potentially significant pharmacological interest.

Original languageEnglish
Pages (from-to)2575-2579
Number of pages5
JournalNeuroReport
Volume7
Issue number15-17
StatePublished - Dec 14 1996
Externally publishedYes

Fingerprint

Combretum
Hiccup
Nuts
Anti-Arrhythmia Agents
Skeletal Muscle Fibers
Sensory Receptor Cells
Membrane Potentials
Action Potentials
Inhibitory Concentration 50
Fruit
Pharmacology
Neurons
Membranes
Neoplasms
combretastatin B-1

Keywords

  • Combretastatin B1
  • Hiccup nut toxin
  • Hydroxybibenzyls
  • K channel blocker
  • Potassium currents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Guatteo, E., Bianchi, L., Faravelli, L., Verotta, L., Pelizzoni, F., Rogers, C. B., & Wanke, E. (1996). A novel K+ channel blocker isolated from 'hiccup nut' toxin. NeuroReport, 7(15-17), 2575-2579.

A novel K+ channel blocker isolated from 'hiccup nut' toxin. / Guatteo, Ezia; Bianchi, Laura; Faravelli, Laura; Verotta, Luisella; Pelizzoni, Francesca; Rogers, Colin B.; Wanke, Enzo.

In: NeuroReport, Vol. 7, No. 15-17, 14.12.1996, p. 2575-2579.

Research output: Contribution to journalArticle

Guatteo, E, Bianchi, L, Faravelli, L, Verotta, L, Pelizzoni, F, Rogers, CB & Wanke, E 1996, 'A novel K+ channel blocker isolated from 'hiccup nut' toxin', NeuroReport, vol. 7, no. 15-17, pp. 2575-2579.
Guatteo E, Bianchi L, Faravelli L, Verotta L, Pelizzoni F, Rogers CB et al. A novel K+ channel blocker isolated from 'hiccup nut' toxin. NeuroReport. 1996 Dec 14;7(15-17):2575-2579.
Guatteo, Ezia ; Bianchi, Laura ; Faravelli, Laura ; Verotta, Luisella ; Pelizzoni, Francesca ; Rogers, Colin B. ; Wanke, Enzo. / A novel K+ channel blocker isolated from 'hiccup nut' toxin. In: NeuroReport. 1996 ; Vol. 7, No. 15-17. pp. 2575-2579.
@article{bbd20c52c5b946c29bfbc77c91e202ce,
title = "A novel K+ channel blocker isolated from 'hiccup nut' toxin",
abstract = "Combretastatin B1, a polyhydroxybibenzyl compound extracted from the fruit of Combretum kraussii, known to contain 'hiccup nut' toxin, reversibly increased the duration, but not the peak or the rate of rise, of the action potential in rat sensory neurones by approximately 300{\%}. This effect was only seen when it was applied to the extracellular side of the membrane. No effects on the resting potential were observed. K+ delayed rectifier currents were inhibited in neurones and in human myotubes with an IC50 of about 300 μM; the HERG-type inward rectifier channels in tumour cells were inhibited to a greater degree. Due to its selective action and the similarity of its blockade to that produced by class III antiarrhythmic drugs, the toxin could be the origin of compounds of potentially significant pharmacological interest.",
keywords = "Combretastatin B1, Hiccup nut toxin, Hydroxybibenzyls, K channel blocker, Potassium currents",
author = "Ezia Guatteo and Laura Bianchi and Laura Faravelli and Luisella Verotta and Francesca Pelizzoni and Rogers, {Colin B.} and Enzo Wanke",
year = "1996",
month = "12",
day = "14",
language = "English",
volume = "7",
pages = "2575--2579",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "15-17",

}

TY - JOUR

T1 - A novel K+ channel blocker isolated from 'hiccup nut' toxin

AU - Guatteo, Ezia

AU - Bianchi, Laura

AU - Faravelli, Laura

AU - Verotta, Luisella

AU - Pelizzoni, Francesca

AU - Rogers, Colin B.

AU - Wanke, Enzo

PY - 1996/12/14

Y1 - 1996/12/14

N2 - Combretastatin B1, a polyhydroxybibenzyl compound extracted from the fruit of Combretum kraussii, known to contain 'hiccup nut' toxin, reversibly increased the duration, but not the peak or the rate of rise, of the action potential in rat sensory neurones by approximately 300%. This effect was only seen when it was applied to the extracellular side of the membrane. No effects on the resting potential were observed. K+ delayed rectifier currents were inhibited in neurones and in human myotubes with an IC50 of about 300 μM; the HERG-type inward rectifier channels in tumour cells were inhibited to a greater degree. Due to its selective action and the similarity of its blockade to that produced by class III antiarrhythmic drugs, the toxin could be the origin of compounds of potentially significant pharmacological interest.

AB - Combretastatin B1, a polyhydroxybibenzyl compound extracted from the fruit of Combretum kraussii, known to contain 'hiccup nut' toxin, reversibly increased the duration, but not the peak or the rate of rise, of the action potential in rat sensory neurones by approximately 300%. This effect was only seen when it was applied to the extracellular side of the membrane. No effects on the resting potential were observed. K+ delayed rectifier currents were inhibited in neurones and in human myotubes with an IC50 of about 300 μM; the HERG-type inward rectifier channels in tumour cells were inhibited to a greater degree. Due to its selective action and the similarity of its blockade to that produced by class III antiarrhythmic drugs, the toxin could be the origin of compounds of potentially significant pharmacological interest.

KW - Combretastatin B1

KW - Hiccup nut toxin

KW - Hydroxybibenzyls

KW - K channel blocker

KW - Potassium currents

UR - http://www.scopus.com/inward/record.url?scp=0029851988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029851988&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 2575

EP - 2579

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 15-17

ER -