A novel four-color PCR assay to assess T-cell receptor gamma gene rearrangements in lymphoproliferative lesions

Francisco Vega, L. Jeffrey Medeiros, Dan Jones, Lynne V. Abruzzo, Raymond Lai, John Manning, Valerie Dunmire, Rajyalakshmi Luthra

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

We describe a novel 4-color polymerase chain reaction (PCR) assay combined with GeneScan analysis to assess for T-cell receptor gamma chain gene (TCRgamma) rearrangements and evaluate its usefulness in 86 lymphoproliferative lesions. In this assay, each variable region (V gamma) family primer is 5′ end-labeled with a different fluorescent dye, allowing determination of the V gamma family involved in each TCRgamma rearrangement. PCR products were analyzed by capillary electrophoresis. We detected clonal TCRgamma rearrangements in 60 (98%) of 61 T-cell lymphomas, 2 (15%) of 13 B-cell lymphomas, and 3 (25%) of 12 reactive lesions. These results compared favorably with conventional PCR methods using denaturing gradient gel electrophoresis, which revealed clonal TCRgamma rearrangements in 37 (90%) of 41 T-cell lymphomas, 1 (25%) of 4 B-cell lymphomas, and 2 (25%) of 8 reactive lesions. This 4-color PCR assay is at least equivalent to conventional PCR methods and is convenient, allows accurate size determination of TCRgamma rearrangements, and identifies the specific V gamma family involved, providing more specific information about TCRgamma rearrangement.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalAmerican journal of clinical pathology
Volume116
Issue number1
DOIs
StatePublished - Jul 1 2001
Externally publishedYes

Keywords

  • 4-color TCRgamma PCR assay
  • High-resolution capillary electrophoresis
  • Polymerase chain reaction
  • T-cell lymphoma
  • T-cell receptor gamma chain gene

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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