A novel cell culture platform for in-vitro enhancement of oxygen delivery leads to improved physiological function of isolated islets of langerhans

C. Fraker, Cherie Stabler, K. Asfura-Gattas, J. Dominguez-Bendala

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In this study, in-vitro experiments were performed on isolated human islets of Langerhans from cadaveric donors. Aliquots of human islet preparations were plated in either conventional polystyrene Petri dishes or perfluroocarbon/ polydimethylsiloxane (PFC/PDMS) devices both at standard (142 mmHg) and physiological core oxygen concentrations, which required 9-12% oxygen (70-80 mm Hg). Twenty-four hours after plating, islets were analyzed for cell loss, glucose response factor and oxygen consumption rate, all indices of beta cell function and viability. Our data show that islets cultured in PFC/PDMS platforms perform significantly better than those kept in regular conditions. Islet survival and function were maximal when adjusting the environmental oxygen concentration to better target the islet physiological range on PFC/PDMS platforms. However, low oxygen concentrations in standard culture vessels resulted in poor function and viability, due to the suboptimal mode of oxygen delivery.

Original languageEnglish (US)
Title of host publication25th Southern Biomedical Engineering Conference 2009
Pages163-164
Number of pages2
DOIs
StatePublished - Nov 6 2009
Event25th Southern Biomedical Engineering Conference 2009 - Miami, FL, United States
Duration: May 15 2009May 17 2009

Publication series

NameIFMBE Proceedings
Volume24
ISSN (Print)1680-0737

Other

Other25th Southern Biomedical Engineering Conference 2009
CountryUnited States
CityMiami, FL
Period5/15/095/17/09

Keywords

  • Hypoxia
  • Perfluorocarbons
  • Polydimethylsiloxane

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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