A nonradioactive assay method for determination of enzymatic activity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)

Subhra Chakrabarti, Sumana Bhattacharya, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A sensitive and nonradioactive assay method for activity determination of Rubisco is described. The method is based on thin-layer chromatographic separation of 3-phosphoglycerate (3-PGA) and D-ribulose-1,5-bisphosphate (RuBP). This assay method allows the quantitative determination of Rubisco activity. Rates of carbon dioxide fixation on RuBP determined by this method were comparable to those obtained independently by other methods. This assay method is reproducible and relatively free from interference.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalJournal of biochemical and biophysical methods
Volume52
Issue number3
DOIs
StatePublished - Aug 1 2002
Externally publishedYes

Keywords

  • Activity determination
  • D-Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)
  • Nonradioactive assay method

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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