A new, specular reflection-based, precorneal tear film stability measurement technique in a rabbit model: Viscoelastic increases tear film stability

Derek Nankivil, Alex Gonzalez, Esdras Arrieta Quintero, Cornelis Rowaan, Mariela C. Aguilar, Krystal Sotolongo, Florence A. Cabot, Sonia H Yoo, Jean-Marie A Parel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

PURPOSE. To develop a safe, noninvasive, noncontact, continuous in vivo method to measure the dehydration rate of the precorneal tear film and to compare the effectiveness of a viscoelastic agent in maintaining the precorneal tear film to that of a balanced salt solution. METHODS. Software was designed to analyze the corneal reflection produced by the operating microscope's coaxial illumination. The software characterized the shape of the reflection, which became distorted as the precorneal tear film evaporated; characterization was accomplished by fitting an ellipse to the reflection and measuring its projected surface area. Balanced salt solution Plus (BSS+) and a 2% hydroxypropylmethylcellulose viscoelastic were used as the test agents. The tear film evaporation rate was characterized and compared over a period of 20 minutes in 20 eyes from 10 New Zealand white rabbits. RESULTS. The ellipse axes ratio and surface area were found to decrease initially after each application of either viscoelastic or BSS+ and then to increase linearly as the tear film began to evaporate (P < 0.001) for eyes treated with BSS+ only. Eyes treated with BSS+ required 7.5 ± 2.7 applications to maintain sufficient corneal hydration during the 20-minute test period, whereas eyes treated with viscoelastic required 1.4 ± 0.5 applications. The rates of evaporation differed significantly (P < 0.043) between viscoelastic and BSS+. CONCLUSIONS. The shape and surface area of the corneal reflection are strongly correlated with the state of the tear film. Rabbits' corneas treated with viscoelastic remained hydrated significantly longer than corneas treated with BSS+.

Original languageEnglish
Pages (from-to)4158-4163
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume55
Issue number7
DOIs
StatePublished - Jun 19 2014

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Tears
Rabbits
Cornea
Software
Salts
Lighting
Dehydration

Keywords

  • Balanced salt solution
  • Corneal hydration
  • Evaporation
  • Precorneal tear film stability
  • Viscoelastic

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

A new, specular reflection-based, precorneal tear film stability measurement technique in a rabbit model : Viscoelastic increases tear film stability. / Nankivil, Derek; Gonzalez, Alex; Arrieta Quintero, Esdras; Rowaan, Cornelis; Aguilar, Mariela C.; Sotolongo, Krystal; Cabot, Florence A.; Yoo, Sonia H; Parel, Jean-Marie A.

In: Investigative Ophthalmology and Visual Science, Vol. 55, No. 7, 19.06.2014, p. 4158-4163.

Research output: Contribution to journalArticle

Nankivil, Derek ; Gonzalez, Alex ; Arrieta Quintero, Esdras ; Rowaan, Cornelis ; Aguilar, Mariela C. ; Sotolongo, Krystal ; Cabot, Florence A. ; Yoo, Sonia H ; Parel, Jean-Marie A. / A new, specular reflection-based, precorneal tear film stability measurement technique in a rabbit model : Viscoelastic increases tear film stability. In: Investigative Ophthalmology and Visual Science. 2014 ; Vol. 55, No. 7. pp. 4158-4163.
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abstract = "PURPOSE. To develop a safe, noninvasive, noncontact, continuous in vivo method to measure the dehydration rate of the precorneal tear film and to compare the effectiveness of a viscoelastic agent in maintaining the precorneal tear film to that of a balanced salt solution. METHODS. Software was designed to analyze the corneal reflection produced by the operating microscope's coaxial illumination. The software characterized the shape of the reflection, which became distorted as the precorneal tear film evaporated; characterization was accomplished by fitting an ellipse to the reflection and measuring its projected surface area. Balanced salt solution Plus (BSS+) and a 2{\%} hydroxypropylmethylcellulose viscoelastic were used as the test agents. The tear film evaporation rate was characterized and compared over a period of 20 minutes in 20 eyes from 10 New Zealand white rabbits. RESULTS. The ellipse axes ratio and surface area were found to decrease initially after each application of either viscoelastic or BSS+ and then to increase linearly as the tear film began to evaporate (P < 0.001) for eyes treated with BSS+ only. Eyes treated with BSS+ required 7.5 ± 2.7 applications to maintain sufficient corneal hydration during the 20-minute test period, whereas eyes treated with viscoelastic required 1.4 ± 0.5 applications. The rates of evaporation differed significantly (P < 0.043) between viscoelastic and BSS+. CONCLUSIONS. The shape and surface area of the corneal reflection are strongly correlated with the state of the tear film. Rabbits' corneas treated with viscoelastic remained hydrated significantly longer than corneas treated with BSS+.",
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AU - Nankivil, Derek

AU - Gonzalez, Alex

AU - Arrieta Quintero, Esdras

AU - Rowaan, Cornelis

AU - Aguilar, Mariela C.

AU - Sotolongo, Krystal

AU - Cabot, Florence A.

AU - Yoo, Sonia H

AU - Parel, Jean-Marie A

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N2 - PURPOSE. To develop a safe, noninvasive, noncontact, continuous in vivo method to measure the dehydration rate of the precorneal tear film and to compare the effectiveness of a viscoelastic agent in maintaining the precorneal tear film to that of a balanced salt solution. METHODS. Software was designed to analyze the corneal reflection produced by the operating microscope's coaxial illumination. The software characterized the shape of the reflection, which became distorted as the precorneal tear film evaporated; characterization was accomplished by fitting an ellipse to the reflection and measuring its projected surface area. Balanced salt solution Plus (BSS+) and a 2% hydroxypropylmethylcellulose viscoelastic were used as the test agents. The tear film evaporation rate was characterized and compared over a period of 20 minutes in 20 eyes from 10 New Zealand white rabbits. RESULTS. The ellipse axes ratio and surface area were found to decrease initially after each application of either viscoelastic or BSS+ and then to increase linearly as the tear film began to evaporate (P < 0.001) for eyes treated with BSS+ only. Eyes treated with BSS+ required 7.5 ± 2.7 applications to maintain sufficient corneal hydration during the 20-minute test period, whereas eyes treated with viscoelastic required 1.4 ± 0.5 applications. The rates of evaporation differed significantly (P < 0.043) between viscoelastic and BSS+. CONCLUSIONS. The shape and surface area of the corneal reflection are strongly correlated with the state of the tear film. Rabbits' corneas treated with viscoelastic remained hydrated significantly longer than corneas treated with BSS+.

AB - PURPOSE. To develop a safe, noninvasive, noncontact, continuous in vivo method to measure the dehydration rate of the precorneal tear film and to compare the effectiveness of a viscoelastic agent in maintaining the precorneal tear film to that of a balanced salt solution. METHODS. Software was designed to analyze the corneal reflection produced by the operating microscope's coaxial illumination. The software characterized the shape of the reflection, which became distorted as the precorneal tear film evaporated; characterization was accomplished by fitting an ellipse to the reflection and measuring its projected surface area. Balanced salt solution Plus (BSS+) and a 2% hydroxypropylmethylcellulose viscoelastic were used as the test agents. The tear film evaporation rate was characterized and compared over a period of 20 minutes in 20 eyes from 10 New Zealand white rabbits. RESULTS. The ellipse axes ratio and surface area were found to decrease initially after each application of either viscoelastic or BSS+ and then to increase linearly as the tear film began to evaporate (P < 0.001) for eyes treated with BSS+ only. Eyes treated with BSS+ required 7.5 ± 2.7 applications to maintain sufficient corneal hydration during the 20-minute test period, whereas eyes treated with viscoelastic required 1.4 ± 0.5 applications. The rates of evaporation differed significantly (P < 0.043) between viscoelastic and BSS+. CONCLUSIONS. The shape and surface area of the corneal reflection are strongly correlated with the state of the tear film. Rabbits' corneas treated with viscoelastic remained hydrated significantly longer than corneas treated with BSS+.

KW - Balanced salt solution

KW - Corneal hydration

KW - Evaporation

KW - Precorneal tear film stability

KW - Viscoelastic

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