A new serum α-amylase assay of high sensitivity

J. John Marshall, Arthur P. Iodice, William J. Whelan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods.

Original languageEnglish
Pages (from-to)277-283
Number of pages7
JournalClinica Chimica Acta
Volume76
Issue number2
DOIs
StatePublished - Apr 15 1977
Externally publishedYes

Fingerprint

Amylases
Assays
Serum
Glucan 1,4-alpha-Glucosidase
Glucose
Substrates
Starch

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

A new serum α-amylase assay of high sensitivity. / Marshall, J. John; Iodice, Arthur P.; Whelan, William J.

In: Clinica Chimica Acta, Vol. 76, No. 2, 15.04.1977, p. 277-283.

Research output: Contribution to journalArticle

Marshall, J. John ; Iodice, Arthur P. ; Whelan, William J. / A new serum α-amylase assay of high sensitivity. In: Clinica Chimica Acta. 1977 ; Vol. 76, No. 2. pp. 277-283.
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