A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity

G. A. Dumanian, W. Dascombe, C. Hong, K. Labadie, K. Garrett, A. S. Sawhney, C. P. Pathak, J. A. Hubbell, P. C. Johnson

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A new nonbiologic photopolymerizable glue, polyethyleneglycol 400 diacrylate, was studied with respect to its mechanical and biochemical interaction with human blood vessels. Using the human placental artery model, we tested the ability of polyethyleneglycol 400 diacrylate to prevent leakage of blood at the site of vascular anastomoses, which are made porous by the presence of tissue gaps and suture puncture sites. Fibrin glue is known to augment local vessel thrombogenicity through the presence of the coagulation enzyme thrombin. We tested the effect of externally applied polyethyleneglycol 400 diacrylate (which does not contain thrombin) on luminal thrombin activity and platelet deposition from flowing human blood. At a shear rate of 312 per second and a transmural pressure of 80 cm H2O, the leakage rate of saline from human placental artery anastomoses was 1.0 ± 1.2 ml/min (n = 8). When the same anastomoses were then glued, 7 of 8 of the anastomoses leaked less than 0.05 ml/min (p < 0.05). Platelet deposition to human vessels was not influenced by the external application of polyethyleneglycol 400 diacrylate either on intact vessels (no polyethyleneglycol 400 diacrylate, 0.51 ± 0.28 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.47 ± 0.26 x 106 platelets/cm2; n = 7) or at anastomoses (no polyethyleneglycol 400 diacrylate, 0.69 ± 0.36 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.53 ± 0.33 x 106 platelets/cm2; n = 8), p > 0.05. Luminal thrombin activity, measured as the rate of fibrinopeptide A formation, was similarly unchanged from control whether polyethyleneglycol 400 diacrylate was applied to intact vessels (no polyethyleneglycol 400 diacrylate, 19 ± 13 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 13 ng fibrinopeptide A/ml; n = 10) or to anastomoses (no polyethyleneglycol 400 diacrylate, 15 ± 12 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 14 ng fibrinopeptide A/ml; n = 10), p > 0.05. We conclude that polyethyleneglycol 400 diacrylate is able to effectively seal vessel puncture sites and anastomotic junctions without acutely augmenting local vascular thrombogenicity. The absence of biologic components and of a need for local delivery of thrombin (as occurs when fibrin glue is used) should prompt additional study of this novel glue.

Original languageEnglish
Pages (from-to)901-907
Number of pages7
JournalPlastic and Reconstructive Surgery
Volume95
Issue number5
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Adhesives
Blood Vessels
Fibrinopeptide A
Thrombin
Fibrin Tissue Adhesive
Punctures
Arteries
polyethyleneglycol 400 diacrylate
Sutures
Blood Platelets
Pressure
Enzymes

ASJC Scopus subject areas

  • Surgery

Cite this

Dumanian, G. A., Dascombe, W., Hong, C., Labadie, K., Garrett, K., Sawhney, A. S., ... Johnson, P. C. (1995). A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity. Plastic and Reconstructive Surgery, 95(5), 901-907.

A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity. / Dumanian, G. A.; Dascombe, W.; Hong, C.; Labadie, K.; Garrett, K.; Sawhney, A. S.; Pathak, C. P.; Hubbell, J. A.; Johnson, P. C.

In: Plastic and Reconstructive Surgery, Vol. 95, No. 5, 01.01.1995, p. 901-907.

Research output: Contribution to journalArticle

Dumanian, GA, Dascombe, W, Hong, C, Labadie, K, Garrett, K, Sawhney, AS, Pathak, CP, Hubbell, JA & Johnson, PC 1995, 'A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity', Plastic and Reconstructive Surgery, vol. 95, no. 5, pp. 901-907.
Dumanian GA, Dascombe W, Hong C, Labadie K, Garrett K, Sawhney AS et al. A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity. Plastic and Reconstructive Surgery. 1995 Jan 1;95(5):901-907.
Dumanian, G. A. ; Dascombe, W. ; Hong, C. ; Labadie, K. ; Garrett, K. ; Sawhney, A. S. ; Pathak, C. P. ; Hubbell, J. A. ; Johnson, P. C. / A new photopolymerizable blood vessel glue that seals human vessel anastomoses without augmenting thrombogenicity. In: Plastic and Reconstructive Surgery. 1995 ; Vol. 95, No. 5. pp. 901-907.
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abstract = "A new nonbiologic photopolymerizable glue, polyethyleneglycol 400 diacrylate, was studied with respect to its mechanical and biochemical interaction with human blood vessels. Using the human placental artery model, we tested the ability of polyethyleneglycol 400 diacrylate to prevent leakage of blood at the site of vascular anastomoses, which are made porous by the presence of tissue gaps and suture puncture sites. Fibrin glue is known to augment local vessel thrombogenicity through the presence of the coagulation enzyme thrombin. We tested the effect of externally applied polyethyleneglycol 400 diacrylate (which does not contain thrombin) on luminal thrombin activity and platelet deposition from flowing human blood. At a shear rate of 312 per second and a transmural pressure of 80 cm H2O, the leakage rate of saline from human placental artery anastomoses was 1.0 ± 1.2 ml/min (n = 8). When the same anastomoses were then glued, 7 of 8 of the anastomoses leaked less than 0.05 ml/min (p < 0.05). Platelet deposition to human vessels was not influenced by the external application of polyethyleneglycol 400 diacrylate either on intact vessels (no polyethyleneglycol 400 diacrylate, 0.51 ± 0.28 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.47 ± 0.26 x 106 platelets/cm2; n = 7) or at anastomoses (no polyethyleneglycol 400 diacrylate, 0.69 ± 0.36 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.53 ± 0.33 x 106 platelets/cm2; n = 8), p > 0.05. Luminal thrombin activity, measured as the rate of fibrinopeptide A formation, was similarly unchanged from control whether polyethyleneglycol 400 diacrylate was applied to intact vessels (no polyethyleneglycol 400 diacrylate, 19 ± 13 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 13 ng fibrinopeptide A/ml; n = 10) or to anastomoses (no polyethyleneglycol 400 diacrylate, 15 ± 12 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 14 ng fibrinopeptide A/ml; n = 10), p > 0.05. We conclude that polyethyleneglycol 400 diacrylate is able to effectively seal vessel puncture sites and anastomotic junctions without acutely augmenting local vascular thrombogenicity. The absence of biologic components and of a need for local delivery of thrombin (as occurs when fibrin glue is used) should prompt additional study of this novel glue.",
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N2 - A new nonbiologic photopolymerizable glue, polyethyleneglycol 400 diacrylate, was studied with respect to its mechanical and biochemical interaction with human blood vessels. Using the human placental artery model, we tested the ability of polyethyleneglycol 400 diacrylate to prevent leakage of blood at the site of vascular anastomoses, which are made porous by the presence of tissue gaps and suture puncture sites. Fibrin glue is known to augment local vessel thrombogenicity through the presence of the coagulation enzyme thrombin. We tested the effect of externally applied polyethyleneglycol 400 diacrylate (which does not contain thrombin) on luminal thrombin activity and platelet deposition from flowing human blood. At a shear rate of 312 per second and a transmural pressure of 80 cm H2O, the leakage rate of saline from human placental artery anastomoses was 1.0 ± 1.2 ml/min (n = 8). When the same anastomoses were then glued, 7 of 8 of the anastomoses leaked less than 0.05 ml/min (p < 0.05). Platelet deposition to human vessels was not influenced by the external application of polyethyleneglycol 400 diacrylate either on intact vessels (no polyethyleneglycol 400 diacrylate, 0.51 ± 0.28 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.47 ± 0.26 x 106 platelets/cm2; n = 7) or at anastomoses (no polyethyleneglycol 400 diacrylate, 0.69 ± 0.36 x 106 platelets/cm2; with polyethyleneglycol 400 diacrylate, 0.53 ± 0.33 x 106 platelets/cm2; n = 8), p > 0.05. Luminal thrombin activity, measured as the rate of fibrinopeptide A formation, was similarly unchanged from control whether polyethyleneglycol 400 diacrylate was applied to intact vessels (no polyethyleneglycol 400 diacrylate, 19 ± 13 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 13 ng fibrinopeptide A/ml; n = 10) or to anastomoses (no polyethyleneglycol 400 diacrylate, 15 ± 12 ng fibrinopeptide A/ml; with polyethyleneglycol 400 diacrylate, 15 ± 14 ng fibrinopeptide A/ml; n = 10), p > 0.05. We conclude that polyethyleneglycol 400 diacrylate is able to effectively seal vessel puncture sites and anastomotic junctions without acutely augmenting local vascular thrombogenicity. The absence of biologic components and of a need for local delivery of thrombin (as occurs when fibrin glue is used) should prompt additional study of this novel glue.

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