Abstract
An algorithm on the fundamental advantages of LC-MS involving high-resolution separation and archiving of data was discussed. A Peak-picking software based on the algorithm was developed. The software was successfully tested in the analysis of a tryptic digest of a mixture of 9 proteins. The advantage of this correlation function approach lie on it's ability to calculate more precisely peak centroids and quantitate the amount of corresponding peptide by using a priori information about the shapes of chromatographic and MS peaks. The algorithm can be used for LC LC(CE)-MALDI-MS in addition to LC (CE)-ESI-MS data.
| Original language | English |
|---|---|
| Title of host publication | Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics |
| Pages | 757-758 |
| Number of pages | 2 |
| State | Published - Dec 1 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country | United States |
| City | Orlando, FL |
| Period | 6/2/02 → 6/6/02 |
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ASJC Scopus subject areas
- Spectroscopy
Cite this
A new peak picking algorithm for LC-MS : Analysis of protein mixture digests. / Andreev, V. P.; Rejtar, T.; Chen, H.; Moskovets, E. V.; Karger, B. L.
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 757-758.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A new peak picking algorithm for LC-MS
T2 - Analysis of protein mixture digests
AU - Andreev, V. P.
AU - Rejtar, T.
AU - Chen, H.
AU - Moskovets, E. V.
AU - Karger, B. L.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - An algorithm on the fundamental advantages of LC-MS involving high-resolution separation and archiving of data was discussed. A Peak-picking software based on the algorithm was developed. The software was successfully tested in the analysis of a tryptic digest of a mixture of 9 proteins. The advantage of this correlation function approach lie on it's ability to calculate more precisely peak centroids and quantitate the amount of corresponding peptide by using a priori information about the shapes of chromatographic and MS peaks. The algorithm can be used for LC LC(CE)-MALDI-MS in addition to LC (CE)-ESI-MS data.
AB - An algorithm on the fundamental advantages of LC-MS involving high-resolution separation and archiving of data was discussed. A Peak-picking software based on the algorithm was developed. The software was successfully tested in the analysis of a tryptic digest of a mixture of 9 proteins. The advantage of this correlation function approach lie on it's ability to calculate more precisely peak centroids and quantitate the amount of corresponding peptide by using a priori information about the shapes of chromatographic and MS peaks. The algorithm can be used for LC LC(CE)-MALDI-MS in addition to LC (CE)-ESI-MS data.
UR - http://www.scopus.com/inward/record.url?scp=2442655451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442655451&partnerID=8YFLogxK
M3 - Conference contribution
SP - 757
EP - 758
BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
ER -