A new peak picking algorithm for LC-MS

Analysis of protein mixture digests

V. P. Andreev, T. Rejtar, H. Chen, E. V. Moskovets, B. L. Karger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An algorithm on the fundamental advantages of LC-MS involving high-resolution separation and archiving of data was discussed. A Peak-picking software based on the algorithm was developed. The software was successfully tested in the analysis of a tryptic digest of a mixture of 9 proteins. The advantage of this correlation function approach lie on it's ability to calculate more precisely peak centroids and quantitate the amount of corresponding peptide by using a priori information about the shapes of chromatographic and MS peaks. The algorithm can be used for LC LC(CE)-MALDI-MS in addition to LC (CE)-ESI-MS data.

Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages757-758
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Proteins
Peptides

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Andreev, V. P., Rejtar, T., Chen, H., Moskovets, E. V., & Karger, B. L. (2002). A new peak picking algorithm for LC-MS: Analysis of protein mixture digests. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 757-758)

A new peak picking algorithm for LC-MS : Analysis of protein mixture digests. / Andreev, V. P.; Rejtar, T.; Chen, H.; Moskovets, E. V.; Karger, B. L.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 757-758.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Andreev, VP, Rejtar, T, Chen, H, Moskovets, EV & Karger, BL 2002, A new peak picking algorithm for LC-MS: Analysis of protein mixture digests. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 757-758, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Andreev VP, Rejtar T, Chen H, Moskovets EV, Karger BL. A new peak picking algorithm for LC-MS: Analysis of protein mixture digests. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 757-758
Andreev, V. P. ; Rejtar, T. ; Chen, H. ; Moskovets, E. V. ; Karger, B. L. / A new peak picking algorithm for LC-MS : Analysis of protein mixture digests. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 757-758
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