A new model of experimental cerebral infarction in New Zealand white rabbits

Zhang Su-ming, Manuel Ramirez-Lassepas, Lucrecia A. Hernandez, Sun Ho Lee, Roberto Heros

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To develop an easy, reproducible experimental model of cerebral infarction (CD without craniotomy in New Zealand white rabbits, a silicone rubber cylinder embedded in a nylon suture was delivered to the middle cerebral arteries through the internal carotid artery in anesthetized animals. Rabbits were sacrificed 0. 5 -5 h after embolization. CI size and location were ascertained by the triphenyl-2H-tetrazolium chloride (TTC) staining method; cerebral blood flow (CBF) was measured prior to and after embolization. in all rabbits after 4 h of ischemia, in 50 % after 3 h and only in 33 % after 2. 5 h. CI did not occur within less than 2. 5 h of ischemia. No correlation was found between size and location of CI and occlusion time. CBF was maximally reduced in the fight MCA territory but was also reduced in both anterior cerebral arteries and left MCA territories. This model is technically easy and the retrievable embolus allows the study of reperfusion by pulling on the nylon suture. It is suitable for studying chemical and molecular changes of the ischemic cells and/or for studying neuroimage changes after ischemic stroke.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalJournal of Tongji Medical University
Volume15
Issue number1
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

    Fingerprint

Keywords

  • CBF
  • embolus
  • experimental cerebral infarction
  • rabbit animal model
  • reperfusion

ASJC Scopus subject areas

  • Management of Technology and Innovation
  • Biomaterials
  • Biomedical Engineering
  • Genetics
  • Biochemistry

Cite this