A new mammalian DNA polymerase with 3′ to 5′ exonuclease activity: DNA polymerase δ

John Byrnes, Kathleen M. Downey, Vicky L. Black, Antero G. So

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

A new species of DNA polymerase has been purified more than 10 000-fold from the cytoplasm of erythroid hyperplastic bone marrow. This DNA polymerase, in contrast to previously described eukaryotic DNA polymerases, is associated with a very active 3′ to 5′ exonuclease activity. Similar to the 3′ to 5′ exonuclease activity associated with prokaryotic DNA polymerases, this enzyme catalyzes the removal of 3′-terminal nucleotides from DNA, as well as a template-dependent conversion of deoxyribonucleoside triphosphates to monophosphates. The exonuclease activity is not separable from the DNA polymerase activity by chromatography on DEAE-Sephadex or hydroxylapatite, and upon sucrose density gradient centrifugation the two activities cosediment at 7 S or at 11 S depending on the ionic strength. Both exonuclease and polymerase activities have identical rates of heat inactivation and both are equally sensitive to hemin and Rifamycin AF/ 013, inhibitors of DNA synthesis that act by binding to DNA polymerase and causing its dissociation from its template/ primer. These results are consistent with the coexistence of two enzyme activities in a single protein.

Original languageEnglish
Pages (from-to)2817-2823
Number of pages7
JournalBiochemistry
Volume15
Issue number13
StatePublished - Dec 1 1976

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Phosphodiesterase I
Exonucleases
DNA-Directed DNA Polymerase
Deoxyribonucleosides
Nucleic Acid Synthesis Inhibitors
DEAE-Dextran
Hemin
Density Gradient Centrifugation
Centrifugation
Enzyme activity
Enzymes
Durapatite
Chromatography
Ionic strength
Osmolar Concentration
Sucrose
Bone
Cytoplasm
Nucleotides
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Byrnes, J., Downey, K. M., Black, V. L., & So, A. G. (1976). A new mammalian DNA polymerase with 3′ to 5′ exonuclease activity: DNA polymerase δ. Biochemistry, 15(13), 2817-2823.

A new mammalian DNA polymerase with 3′ to 5′ exonuclease activity : DNA polymerase δ. / Byrnes, John; Downey, Kathleen M.; Black, Vicky L.; So, Antero G.

In: Biochemistry, Vol. 15, No. 13, 01.12.1976, p. 2817-2823.

Research output: Contribution to journalArticle

Byrnes, J, Downey, KM, Black, VL & So, AG 1976, 'A new mammalian DNA polymerase with 3′ to 5′ exonuclease activity: DNA polymerase δ', Biochemistry, vol. 15, no. 13, pp. 2817-2823.
Byrnes, John ; Downey, Kathleen M. ; Black, Vicky L. ; So, Antero G. / A new mammalian DNA polymerase with 3′ to 5′ exonuclease activity : DNA polymerase δ. In: Biochemistry. 1976 ; Vol. 15, No. 13. pp. 2817-2823.
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