A new experimental model of contusion in the rat. Histopathological analysis and temporal patterns of cerebral blood flow disturbances

Peter Mathew, Ross Bullock, David I. Graham, William L. Maxwell, Graham M. Teasdale, James McCulloch

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The authors have devised a simple reproducible rodent model of focal cortical injury that uses a mechanical suction force applied through intact dura. The time course and pattern of changes in neurons, glia, and microvasculature were investigated using this model. Early traumatic disruption of the blood-brain barrier and hemorrhage do not occur in this model; however, many of the features of human confusion seen with light and electron microscopy are closely reproduced. At the site of injury, early swelling and lucency of neural dendritic processes have been shown to precede an astrocyte response. In the absence of perivascular hemorrhage, delayed perivascular protein leakage and polymorphonuclear infiltration of the damage cortex occurs, which is suggestive of an acute inflammatory response. Cerebral blood flow (CBF) has been measured using 14C-iodoantipyrine autoradiography at 30 minutes, 4 hours, and 24 hours after induction of negative-pressure injury in rats anesthetized with halothane and in time- matched sham-operated controls. A significant reduction n blood flow in the sensorimotor cortex at the site of the injury was present at 30 minutes, 4 hours, and 24 hours after induction of the lesion, compared to the contralateral cortex (superficial lamina, ipsilateral 50 ± 7 ml/100 g/minute, contralateral 112 ± 26 ml/100 g/minute). The CBF was significantly reduced at the ipsilateral entorhinal cortex at 30 minutes postinjury but not significant reduction was demonstrated at later points. Although marked alterations in CBF occurred in this cortical injury model, the magnitude and duration of the reduction in CBF are not consistent with those necessary for production of ischemic cell damage. These data indicate that this model of cortical injury can be used to examine biochemical aspects of confusion without domination by ischemic pathophysiology.

Original languageEnglish (US)
Pages (from-to)860-870
Number of pages11
JournalJournal of neurosurgery
Volume85
Issue number5
DOIs
StatePublished - Nov 1996

Keywords

  • focal injury
  • inflammation
  • ischemia
  • rat

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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