A new atomic force microscopy technique for the measurement of the elastic properties of biological materials

W. Xu, P. J. Mulhern, B. L. Blackford, M. H. Jericho, I. Templeton, D. C. Dahn, Vincent T. Moy, D. A. Chernoff

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We developed a new technique to measure elastic properties by using the atomic force microscope (AFM) tip to press samples into grooves etched in a GaAs substrate. We measured the Young's modulus of β-chitin fibres with cross-sections less than 40 nm x 20 nm to be 1-2 x 1011 N/m2. In the isotropic approximation, the Young's modulus of the S-layer sheath of the archaeobacterium Methanospirillum hungatei was 1-3 x 1010 N/m2. By testing the sheath to breaking strength we estimated the bacterium can sustain an internal pressure as high as 100-200 atmospheres (1-2 x 107 N/m2).

Original languageEnglish (US)
Pages (from-to)499-506
Number of pages8
JournalScanning Microscopy
Volume8
Issue number3
StatePublished - Dec 1 1994
Externally publishedYes

Fingerprint

sheaths
Biological materials
Atomic force microscopy
modulus of elasticity
elastic properties
Elastic moduli
atomic force microscopy
chitin
Chitin
internal pressure
grooves
bacteria
Bacteria
Microscopes
microscopes
atmospheres
fibers
Fibers
cross sections
Testing

Keywords

  • Atomic force microscopy (AFM)
  • chitin
  • elasticity
  • force
  • internal pressure
  • Methanospirillum hungatei

ASJC Scopus subject areas

  • Instrumentation

Cite this

Xu, W., Mulhern, P. J., Blackford, B. L., Jericho, M. H., Templeton, I., Dahn, D. C., ... Chernoff, D. A. (1994). A new atomic force microscopy technique for the measurement of the elastic properties of biological materials. Scanning Microscopy, 8(3), 499-506.

A new atomic force microscopy technique for the measurement of the elastic properties of biological materials. / Xu, W.; Mulhern, P. J.; Blackford, B. L.; Jericho, M. H.; Templeton, I.; Dahn, D. C.; Moy, Vincent T.; Chernoff, D. A.

In: Scanning Microscopy, Vol. 8, No. 3, 01.12.1994, p. 499-506.

Research output: Contribution to journalArticle

Xu, W, Mulhern, PJ, Blackford, BL, Jericho, MH, Templeton, I, Dahn, DC, Moy, VT & Chernoff, DA 1994, 'A new atomic force microscopy technique for the measurement of the elastic properties of biological materials', Scanning Microscopy, vol. 8, no. 3, pp. 499-506.
Xu W, Mulhern PJ, Blackford BL, Jericho MH, Templeton I, Dahn DC et al. A new atomic force microscopy technique for the measurement of the elastic properties of biological materials. Scanning Microscopy. 1994 Dec 1;8(3):499-506.
Xu, W. ; Mulhern, P. J. ; Blackford, B. L. ; Jericho, M. H. ; Templeton, I. ; Dahn, D. C. ; Moy, Vincent T. ; Chernoff, D. A. / A new atomic force microscopy technique for the measurement of the elastic properties of biological materials. In: Scanning Microscopy. 1994 ; Vol. 8, No. 3. pp. 499-506.
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